Comparative Analysis of the Degree of Hydrolysis of Biopolymers in Hyaline Cartilage Homogenates in the Presence of Proteolytic Enzymes

Abstract

The study of the degree of hydrolysis depending on the action of various proteolytic enzymes is one of the tasks in the development of nutraceuticals from connective tissues for biomedicine. Hydrolysis of biopolymers in hyaline cartilage homogenates from the trachea of cattle and pigs was carried out under the action of the enzymes pancreatin, chymopsin, papain, and the proteolytic drug karipazim containing papain. It has been shown that karipazim produced by MedFlorina acted more effectively than karipazim produced by Vifitech, and the degree of collagen hydrolysis was maximal at 60°C and a concentration of karipazim of 10%. A more complete hydrolysis of proteoglycans was observed in the homogenates of hyaline cartilage of cattle, since glucose, the final product of glycosaminoglycan hydrolysis, was identified on NMR spectra.