革命性的抗菌表面:通过双光子聚合技术三维打印纳米级和微米级拓扑结构

Ning Tan, Jisun Im, Nigel Neate, Ricky D. Wildman, Georgina Elizabeth Marsh, M. Yee
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引用次数: 0

摘要

由于抗菌剂的过度使用和滥用,细菌对抗菌剂的防御机制不断演变,导致了一个灾难性的问题--抗菌剂耐药性,这促使人们寻求创新的抗菌方法,在不使用任何化学物质的情况下有效抑制细菌生长。受天然纳米形貌(如蝉翼上的纳米形貌)的启发,量身定制的纳米和微观结构因其独特的机械抗菌特性而在抗菌活性方面大有可为。在各种纳米/微制造技术中,双光子聚合(TPP)技术是一种多功能、精确的方法,可制造出具有亚微米分辨率的任意功能三维结构。该过程包括使用飞秒激光脉冲诱导生物相容性丙烯酸酯基光敏树脂以精确的空间模式聚合,从而生成纳米/微结构。在这项研究中,我们研究了激光功率、曝光时间和界面值等关键制造参数对实现最终预定义纳米/微结构的影响。显微镜分析表明,成功制备的纳米结构高度在 350-650 nm 之间,直径在 300-400 nm 之间,中心到中心的距离在 700-2000 nm 之间。我们针对医疗机构中常见的铜绿假单胞菌病原菌测试了两种光子设计的纳米结构的机械抗菌可行性。我们的研究结果表明,TPP 纳米/微结构通过纳米/微结构与细菌之间的物理-机械相互作用,创造出具有杀菌活性的表面,从而表现出令人感兴趣的抗菌活性。这项研究为纳米技术和生物医学领域的先进抗菌应用铺平了道路,为抗击抗菌药耐药性和促进全球健康做出了重要贡献。
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Revolutionizing Antibacterial Surfaces: 3D Printed Nanoscale and Microscale Topographies through Two-Photon Polymerization
The evolving bacteria defense mechanism against antimicrobial agents due to the overuse and misuse of antimicrobial chemicals has led to a catastrophic problem - antimicrobial resistance, this has spurred the quest for innovative antibacterial approach to inhibit bacterial growth effectively without using any chemicals. Tailored nano- and microstructured architecture, inspired by natural nanotopography such as those found on cicada wings, hold great promise in antibacterial activity due to their unique mechano-antibacterial properties. Among the various nano-/microfabrication techniques, the two-photon polymerisation (TPP) stands out as a versatile and precise approach to fabricate arbitrarily functional three-dimensional structures with sub-micrometre resolution. The process involves the use of femtosecond laser pulses to induce polymerization of a biocompatible acrylate-based photoresin in a precise spatial pattern to generate the nano-/microarchitecture. In this study, we investigated the influence of key fabrication parameters, such as laser power, exposure time, and interface value to achieve the final pre-defined nano-/microarchitecture. Microscopy analysis showed that nanostructure of heights between 350-650 nm; 300-400 nm diameter; and increasing center-to-center distances of 700-2000 nm were successfully fabricated. The mechano-antibacterial feasibility of the two photon-designed nanoarchitecture were tested against P. aeruginosa pathogenic bacteria commonly encountered in healthcare settings. Our results showed that the TPP nano-/microarchitecture demonstrated intriguing antibacterial activity through physico-mechanical interactions between the nano-/microarchitectures and bacteria, creating surfaces that exhibit bactericidal activity. This study paves the way for advanced antibacterial applications in the field of nanotechnology and biomedicine, making a significant contribution to the ongoing efforts in combating antimicrobial resistance and promoting global health.
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