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引用次数: 0
摘要
调节性 T 细胞(Tregs)是 T 细胞的一个特化亚群,具有免疫调节和调节的双重功能。最近的研究报告指出,调节性 T 细胞可介导免疫反应,并调节骨和心肌等非淋巴组织的发育和修复过程。此外,Tregs 还能促进受损肺组织的修复和再生。然而,有关 Tregs 在肺发育中作用的研究还很有限。本研究旨在通过研究Tregs及其标志性细胞因子叉头盒P3(Foxp3)在小鼠肺发育不同阶段的动态变化,并建立抗CD25抗体诱导的Treg耗竭小鼠模型,评估Tregs在肺发育中的作用。在小鼠肺发育的早期阶段,尤其是管状期和囊状期,Treg的丰度、Foxp3和转化生长因子-β(TGF-β)的表达水平均上调。这与肺泡上皮细胞和血管内皮细胞的增殖期相吻合,表明了对肺部动态发育过程的适应。此外,Tregs的消耗破坏了肺组织形态,并下调了肺发育相关因子,如表面活性蛋白C(SFTPC)、血管内皮生长因子A(VEGFA)和血小板内皮细胞粘附分子-1(PECAM1/CD31)。这些研究结果表明,Tregs 能促进小鼠肺部发育。
Role of regulatory T cells in mouse lung development
Regulatory T cells (Tregs) constitute a specialized subset of T cells with dual immunoregulatory and modulatory functions. Recent studies have reported that Tregs mediate immune responses and regulate the development and repair processes in non-lymphoid tissues, including bone and cardiac muscle. Additionally, Tregs facilitate the repair and regeneration of damaged lung tissues. However, limited studies have examined the role of Tregs in pulmonary development. This study aimed to evaluate the role of Tregs in pulmonary development by investigating the dynamic alterations in Tregs and their hallmark cellular factor Forkhead box P3 (Foxp3) at various stages of murine lung development and establishing a murine model of anti-CD25 antibody-induced Treg depletion. During the early stages of murine lung development, especially the canalicular and saccular stages, the levels of Treg abundance and expression of Foxp3 and transforming growth factor-β (TGF-β) were upregulated. This coincided with the proliferation period of alveolar epithelial cells and vascular endothelial cells, indicating an adaptation to the dynamic lung developmental processes. Furthermore, the depletion of Tregs disrupted lung tissue morphology and downregulated lung development-related factors, such as surfactant protein C (SFTPC), vascular endothelial growth factor A (VEGFA) and platelet endothelial cell adhesion molecule-1 (PECAM1/CD31). These findings suggest that Tregs promote murine lung development.
期刊介绍:
Experimental Biology and Medicine (EBM) is a global, peer-reviewed journal dedicated to the publication of multidisciplinary and interdisciplinary research in the biomedical sciences. EBM provides both research and review articles as well as meeting symposia and brief communications. Articles in EBM represent cutting edge research at the overlapping junctions of the biological, physical and engineering sciences that impact upon the health and welfare of the world''s population.
Topics covered in EBM include: Anatomy/Pathology; Biochemistry and Molecular Biology; Bioimaging; Biomedical Engineering; Bionanoscience; Cell and Developmental Biology; Endocrinology and Nutrition; Environmental Health/Biomarkers/Precision Medicine; Genomics, Proteomics, and Bioinformatics; Immunology/Microbiology/Virology; Mechanisms of Aging; Neuroscience; Pharmacology and Toxicology; Physiology; Stem Cell Biology; Structural Biology; Systems Biology and Microphysiological Systems; and Translational Research.