将芽孢杆菌乳化/交联封装在淀粉/PVA 基微颗粒中,用于农业应用

IF 2.3 Q1 AGRICULTURE, MULTIDISCIPLINARY ACS agricultural science & technology Pub Date : 2024-03-18 DOI:10.1021/acsagscitech.4c00029
Marina Momesso Lopes, Ludimila Araújo Lodi, Christiane Abreu de Oliveira-Paiva and Cristiane Sanchez Farinas*, 
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引用次数: 0

摘要

使用芽孢杆菌促进植物生长的一个主要缺点是,在不利的田间条件下和贮藏期间会失去活力。在此,我们提出了一种利用乳液/交联技术与基于淀粉/聚乙烯醇(PVA)的基质进行封装的策略,以提高巨大芽孢杆菌的细胞活力。交联剂三偏磷酸钠(STMP)与淀粉(ST)或蒙脱石(MMT)结合可形成微颗粒(分别为 ST/PVA-STMP + ST 和 ST/PVA-STMP + MMT)。表征采用了傅立叶变换红外光谱(FTIR)、X 射线衍射(XRD)和扫描电子显微镜(SEM)。两种微粒在封装后的细胞存活率均高于 10.75 log10 CFU g-1。当暴露在热和杀真菌剂的压力下时,微颗粒显示出保护作用,将细胞活力维持在 9.5 log10 CFU g-1 左右。封装在加速货架期试验(ASLT)中也被证明具有优势,符合不同国家的商业化要求。这些发现凸显了封装程序在扩大微生物接种剂在可持续农业中的应用方面的潜力。
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Emulsion/Cross-Linking Encapsulation of Bacillus in Starch/PVA-Based Microparticles for Agricultural Applications

A major drawback of using Bacillus in the promotion of plant growth is the loss of viability under adverse field conditions and during storage. Here, we propose an encapsulation strategy using an emulsion/cross-linking technique with a starch/poly(vinyl alcohol) (PVA)-based matrix for enhancing the cell viability of Bacillus megaterium. The cross-linking agent, trisodium trimetaphosphate (STMP), combined with either starch (ST) or montmorillonite (MMT), allowed the formation of microparticles (ST/PVA-STMP + ST and ST/PVA-STMP + MMT, respectively). Characterization was performed using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Both microparticles exhibited cell viability higher than 10.75 log10 CFU g–1 after the encapsulation procedure. When exposed to heat and fungicide stresses, the microparticles showed a protective role, maintaining cell viability around 9.5 log10 CFU g–1. The encapsulation also proved advantageous in the accelerated shelf-life test (ASLT) assay, meeting the commercialization requirements of different countries. These findings highlight the potential of the encapsulation procedure to expand the use of microbial inoculants for sustainable agriculture.

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