来自熊蜂的 Fructobacillus evanidus sp.

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2024-03-22 DOI:10.1016/j.syapm.2024.126505
Juliana Botero , Charlotte Peeters , Evelien De Canck , David Laureys , Anneleen D. Wieme , Ilse Cleenwerck , Eliza Depoorter , Jessy Praet , Denis Michez , Guy Smagghe , Peter Vandamme
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引用次数: 0

摘要

对蜜蜂微生物组研究的增加是由于对全球授粉者减少的担忧。大黄蜂寄生的核心和非核心微生物群可能有助于提高其一生的适应能力。大黄蜂工蜂肠道微生物组中果酸杆菌的存在,或果酸杆菌对核心共生菌的替代,一直被认为是菌群失调的标志。对从大黄蜂中分离出的 21 个果酸杆菌基因组进行的系统发生组分析和功能基因组鉴定表明,它们代表了 4 个物种,即卡氏果酸杆菌(Fructobacillus cardui)、果寡糖果酸杆菌(Fructobacillus fructosus)、滋养果酸杆菌(Fructobacillus tropaeoli)和新物种埃瓦尼德果酸杆菌(Fructobacillus evanidus sp.nov)。我们的研究结果证实了两个在系统发育和功能上截然不同的果酸杆菌物种支系的存在,它们在基因组大小、G + C 含量百分比、编码 DNA 序列数量和代谢特征上都有所不同。支系 1 和支系 2 的物种在氨基酸代谢方面存在差异,在碳水化合物代谢方面差异较小,其中 F. evanidus 和 F. tropaeoli 的基因组具有较多的完整代谢途径。虽然果杆菌基因组编码的基因可以实现粘附、生物膜形成、抗菌活性和解毒,但从大黄蜂肠道中分离出的其他细菌似乎更适合与大黄蜂宿主共存。本研究从几种大黄蜂肠道样本中分离并鉴定出多种果酸杆菌,这也证明果酸杆菌与大黄蜂宿主之间并不存在特定的伙伴关系。
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A comparative genomic analysis of Fructobacillus evanidus sp. nov. from bumble bees

The increase in studies on bee microbiomes is prompted by concerns over global pollinator declines. Bumble bees host core and non-core microbiota which may contribute to increased lifetime fitness. The presence of Fructobacillus in the gut microbiomes of bumble bee workers, or the replacement of core symbionts with Fructobacillus bacteria, has been considered a marker of dysbiosis. A phylogenomic analysis and functional genomic characterization of the genomes of 21 Fructobacillus isolates from bumble bees demonstrated that they represented four species, i.e. Fructobacillus cardui, Fructobacillus fructosus, Fructobacillus tropaeoli, and the novel species Fructobacillus evanidus sp. nov. Our results confirmed and substantiated the presence of two phylogenetically and functionally distinct Fructobacillus species clades that differ in genome size, percentage G + C content, the number of coding DNA sequences and metabolic characteristics. Clade 1 and clade 2 species differed in amino acid and, to a lesser extent, in carbohydrate metabolism, with F. evanidus and F. tropaeoli genomes featuring a higher number of complete metabolic pathways. While Fructobacillus genomes encoded genes that allow adhesion, biofilm formation, antibacterial activity and detoxification, other bacteria isolated from the bumble bee gut appeared better equipped to co-exist with the bumble bee host. The isolation and identification of multiple Fructobacillus species from several bumble bee gut samples in the present study also argued against a specific partnership between Fructobacillus species and their bumble bee hosts.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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