C. Rowlands, Ke Guo, P. Pantazis, Konstantinos Kalyviotis
{"title":"高光谱斜面显微镜","authors":"C. Rowlands, Ke Guo, P. Pantazis, Konstantinos Kalyviotis","doi":"10.1117/12.3003078","DOIUrl":null,"url":null,"abstract":"A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos","PeriodicalId":517893,"journal":{"name":"High-Speed Biomedical Imaging and Spectroscopy IX","volume":"4 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hyperspectral oblique plane microscopy\",\"authors\":\"C. Rowlands, Ke Guo, P. Pantazis, Konstantinos Kalyviotis\",\"doi\":\"10.1117/12.3003078\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos\",\"PeriodicalId\":517893,\"journal\":{\"name\":\"High-Speed Biomedical Imaging and Spectroscopy IX\",\"volume\":\"4 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"High-Speed Biomedical Imaging and Spectroscopy IX\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1117/12.3003078\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"High-Speed Biomedical Imaging and Spectroscopy IX","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.3003078","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos
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