经过验证的限制性酶 ddPCR cg05575921 (AHRR) 检测法可准确评估吸烟暴露。

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL ACS Applied Energy Materials Pub Date : 2024-03-25 DOI:10.1186/s13148-024-01659-1

Sandra Fitzgerald, Basharat Bhat, Cristin Print, Gregory T Jones

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引用次数: 0

摘要

背景与方法：本研究针对 AHRR 基因体中的 cg005575921 设计了一种新型的基于限制性酶（RE）消化的液滴数字聚合酶链反应（ddPCR）检测方法，并将其与亚硫酸氢盐转换（BIS）ddPCR 和 Illumina DNA 甲基化阵列的匹配结果进行了比较：结果：RE ddPCR cg05575921 检测与 BIS ddPCR 检测结果一致（r2 = 0.94，P 结论：RE ddPCR cg05575921 检测结果与 BIS ddPCR 检测结果一致：RE ddPCR cg05575921 检测能准确预测吸烟状况，可作为 "精准医疗 "慢性病风险筛查工具的有用组成部分。

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A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure.

Background & methods: In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array.

Results: The RE ddPCR cg05575921 assay appeared concordant with BIS ddPCR (r² = 0.94, P < 0.0001) and, when compared with the Illumina array, had significantly better smoking status classification performance for current versus never smoked (AUC 0.96 versus 0.93, P < 0.04) and current versus ex-smoker (AUC 0.88 versus 0.83, P < 0.04) comparisons.

Conclusions: The RE ddPCR cg05575921 assay accurately predicts smoking status and could be a useful component of 'precision-medicine' chronic disease risk screening tools.

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.