Bart Weijts, Eugene Tkachenko, David Traver, Alex Groisman
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引用次数: 0
摘要
我们介绍了用于斑马鱼幼体躯干成像的四孔皿的设计、制造和应用。该培养皿便于固定麻醉斑马鱼幼体,将其尾部轻轻压在显微镜盖玻片上,从而使用高分辨率物镜对受精后 24-72 h 的斑马鱼进行纵向成像。
A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish.
We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.