Silvia Galli, Gem Flint, Lucie Růžičková and Marco Di Antonio
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引用次数: 0
摘要
能够在全基因组范围内绘制染色质中 DNA G-四叠体结构图的方法的开发,在为活细胞中这些结构的形成提供证据方面发挥了至关重要的作用。过去十年间,文献中报道了多种旨在绘制 G 型四联体结构图的方法。在这篇重要综述中,我们试图从技术上概述目前可用的基因组学方法的相对优缺点,为评估实验需求和选择最合适的方法提供逐步指导,以实现有效的 DNA G-四联体全基因组图谱绘制。
Genome-wide mapping of G-quadruplex DNA: a step-by-step guide to select the most effective method
The development of methods that enabled genome-wide mapping of DNA G-quadruplex structures in chromatin has played a critical role in providing evidence to support the formation of these structures in living cells. Over the past decade, a variety of methods aimed at mapping G-quadruplexes have been reported in the literature. In this critical review, we have sought to provide a technical overview on the relative strengths and weaknesses of the genomics approaches currently available, offering step-by-step guidance to assessing experimental needs and selecting the most appropriate method to achieve effective genome-wide mapping of DNA G-quadruplexes.