通过分光光度法快速简便地量化甲壳素纳米须和纳米纤维表面的氨基基团

IF 2.3 4区 化学 Q3 POLYMER SCIENCE Polymer Journal Pub Date : 2024-03-25 DOI:10.1038/s41428-024-00904-x
Jun Araki, Shiori Yoda, Riku Kudo
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摘要

利用三种氨基标记试剂(2,4,6-三硝基苯磺酸钠盐(TNBS)、邻苯二甲醛(OPA)和茚三酮)和两种阳离子染料(活性红 4(RR4)和酸性橙 7(AO7)),通过分光光度法对甲壳素纳米须和甲壳素纳米纤维上的表面氨基(SAG)进行了快速简便的定量分析。与 SAG 结合后,通过分光光度法测定过量 TNBS 和 OPA 的量,并从初始量中减去。在 ChNWs/ChNFs 中加入茚三酮后,会生成鲁海曼氏紫色产物,并通过分光光度法进行定量。在 ChNWs/ChNFs 的 SAG 中过量添加 RR4 和 AO7 时,这些染料会吸附在表面氨基上,过量的量同样会被量化。每种方法得出的氨基含量都不同,并与滴定值进行比较。虽然用标记试剂得到的数值被大大低估,但用 TNBS 和茚三酮得到的数值与滴定法得到的数值成正比。在 pH 值为 2 或 3 时使用 AO7 吸附得到的值与 ChNW 的滴定值非常吻合,但 ChNF 则不然。使用前两种标记试剂与转换方程或使用 AO7 吸附均可获得可靠的结果。
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Rapid and facile quantification of surface amino groups on chitin nanowhiskers and nanofibers via spectrophotometry
Surface amino groups (SAGs) on chitin nanowhiskers and chitin nanofibers were rapidly and facilely quantified via spectrophotometry using three amino-labeling reagents (2,4,6-trinitrobenzensulfonic acid sodium salt (TNBS), o-phthalaldehyde (OPA) and ninhydrin) and two cationic dyes (reactive red 4 (RR4) and acid orange 7 (AO7)). After binding to the SAGs, the amounts of excess TNBS and OPA were determined via spectrophotometry and subtracted from the initial quantity. After ninhydrin was added to ChNWs/ChNFs, Ruhemann’s purple product was generated, which was quantified via spectrophotometry. When RR4 and AO7 were added in excess to the SAG of ChNWs/ChNFs, these dyes were adsorbed onto surface amino groups, and the excess amounts were similarly quantified. Each method yielded different amino group contents, which were compared with the titration values. Although the values obtained by labeling reagents were considerably underestimated, those obtained using TNBS and ninhydrin were proportional to those obtained by titration. The values obtained using AO7 adsorption at pH 2 or 3 corresponded well with the titration values for ChNWs but not ChNFs. Reliable results were attained using the two former labeling reagents with conversion equations or using AO7 adsorption. Surface amino groups (SAGs) on nanochitin materials were quantified using three amino-labeling reagents and two cationic dyes. After binding to SAGs, the excess labeling reagents or generated molecules were assessed by spectrophotometry. The dyes were adsorbed onto SAGs, and the excess was similarly quantified. The obtained values were compared with the titration values. Although the values by labeling reagents were underestimated, some of the values were proportional to those by titration. Reliable results were attained using the two labeling reagents with conversion equations or using Acid Orange 7 adsorption.
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来源期刊
Polymer Journal
Polymer Journal 化学-高分子科学
CiteScore
5.60
自引率
7.10%
发文量
131
审稿时长
2.5 months
期刊介绍: Polymer Journal promotes research from all aspects of polymer science from anywhere in the world and aims to provide an integrated platform for scientific communication that assists the advancement of polymer science and related fields. The journal publishes Original Articles, Notes, Short Communications and Reviews. Subject areas and topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Polymer synthesis and reactions Polymer structures Physical properties of polymers Polymer surface and interfaces Functional polymers Supramolecular polymers Self-assembled materials Biopolymers and bio-related polymer materials Polymer engineering.
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