建立生成和维持雌激素受体阳性乳腺癌类器官模型的条件

IF 6.1 1区 医学 Q1 ONCOLOGY Breast Cancer Research Pub Date : 2024-03-29 DOI:10.1186/s13058-024-01798-6
Michael U J Oliphant, Dipikaa Akshinthala, Senthil K. Muthuswamy
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引用次数: 0

摘要

雌激素受体阳性(ER+)乳腺癌的患者衍生类器官模型将为更好地了解耐药性和疾病进展提供急需的工具。然而,如何在体外建立并长期保持ER的表达、功能和反应仍然是一项重大挑战。在这里,我们报告了一种ER+乳腺肿瘤类器官培养基(BTOM-ER)的开发情况,它能保持ER的表达、雌激素反应性和依赖性,以及对ER+患者异种移植类器官(PDXO)内分泌治疗的敏感性。我们的研究结果表明,亚型特异性培养条件能更好地模拟乳腺上皮生物学特性和微环境,为研究ER+乳腺癌的治疗反应和疾病进展提供了一个强大的平台。
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Establishing conditions for the generation and maintenance of estrogen receptor-positive organoid models of breast cancer
Patient-derived organoid models of estrogen receptor-positive (ER+) breast cancer would provide a much-needed tool to understand drug resistance and disease progression better. However, the establishment and long-term maintenance of ER expression, function, and response in vitro remains a significant challenge. Here, we report the development of an ER+ breast tumor organoid medium (BTOM-ER) that conserves ER expression, estrogen responsiveness, and dependence, as well as sensitivity to endocrine therapy of ER+ patient-derived xenograft organoids (PDXO). Our findings demonstrate the utility of subtype-specific culture conditions that better mimic the characteristics of the breast epithelial biology and microenvironment, providing a powerful platform for investigating therapy response and disease progression of ER+ breast cancer.
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来源期刊
Breast Cancer Research
Breast Cancer Research 医学-肿瘤学
自引率
0.00%
发文量
76
期刊介绍: Breast Cancer Research is an international, peer-reviewed online journal, publishing original research, reviews, editorials and reports. Open access research articles of exceptional interest are published in all areas of biology and medicine relevant to breast cancer, including normal mammary gland biology, with special emphasis on the genetic, biochemical, and cellular basis of breast cancer. In addition to basic research, the journal publishes preclinical, translational and clinical studies with a biological basis, including Phase I and Phase II trials.
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