Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam
{"title":"从孟加拉国两个地区的养鸡场中发现的弯曲杆菌属抗菌药耐药性概况。","authors":"Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam","doi":"10.1089/fpd.2023.0079","DOIUrl":null,"url":null,"abstract":"<p><p>The rapid emergence of antimicrobial resistance (AMR) in <i>Campylobacter</i> has reinforced its status as a foodborne pathogen of significant public health concern. Resistant <i>Campylobacter</i> is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in <i>Campylobacter</i> spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR <i>Campylobacter</i> across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for <i>Campylobacter</i> spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in <i>Campylobacter</i>-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were <i>Campylobacter</i>-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant <i>Campylobacter</i> were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including <i>bla</i><sub>TEM</sub> (in 97.1% of isolates), <i>strA</i>-<i>strB</i> (85.9%), <i>tetA</i> (70.6%), <i>tetB</i> (32.4%), <i>qnrS</i> (23.5%), <i>bla</i><sub>CTX-M-1</sub> (20.6%), <i>qnrB</i> (20.6%), <i>bla</i><sub>SHV</sub> (8.8%), <i>aadB</i> (5.9%), and <i>qnrA</i> (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in <i>Campylobacter</i> isolates was significantly (<i>p</i> ≤ 0.05) associated with the presence of <i>bla</i><sub>TEM</sub>, <i>tetA</i>, and <i>bla</i><sub>SHV</sub> genes, respectively. The high rates of AMR in <i>Campylobacter</i> isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived <i>Campylobacter</i> isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial Resistance Profiles of <i>Campylobacter</i> spp. Recovered from Chicken Farms in Two Districts of Bangladesh.\",\"authors\":\"Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam\",\"doi\":\"10.1089/fpd.2023.0079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The rapid emergence of antimicrobial resistance (AMR) in <i>Campylobacter</i> has reinforced its status as a foodborne pathogen of significant public health concern. Resistant <i>Campylobacter</i> is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in <i>Campylobacter</i> spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR <i>Campylobacter</i> across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for <i>Campylobacter</i> spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in <i>Campylobacter</i>-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were <i>Campylobacter</i>-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant <i>Campylobacter</i> were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including <i>bla</i><sub>TEM</sub> (in 97.1% of isolates), <i>strA</i>-<i>strB</i> (85.9%), <i>tetA</i> (70.6%), <i>tetB</i> (32.4%), <i>qnrS</i> (23.5%), <i>bla</i><sub>CTX-M-1</sub> (20.6%), <i>qnrB</i> (20.6%), <i>bla</i><sub>SHV</sub> (8.8%), <i>aadB</i> (5.9%), and <i>qnrA</i> (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in <i>Campylobacter</i> isolates was significantly (<i>p</i> ≤ 0.05) associated with the presence of <i>bla</i><sub>TEM</sub>, <i>tetA</i>, and <i>bla</i><sub>SHV</sub> genes, respectively. The high rates of AMR in <i>Campylobacter</i> isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived <i>Campylobacter</i> isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.</p>\",\"PeriodicalId\":12333,\"journal\":{\"name\":\"Foodborne pathogens and disease\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Foodborne pathogens and disease\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1089/fpd.2023.0079\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2023.0079","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Antimicrobial Resistance Profiles of Campylobacter spp. Recovered from Chicken Farms in Two Districts of Bangladesh.
The rapid emergence of antimicrobial resistance (AMR) in Campylobacter has reinforced its status as a foodborne pathogen of significant public health concern. Resistant Campylobacter is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in Campylobacter spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR Campylobacter across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for Campylobacter spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in Campylobacter-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were Campylobacter-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant Campylobacter were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including blaTEM (in 97.1% of isolates), strA-strB (85.9%), tetA (70.6%), tetB (32.4%), qnrS (23.5%), blaCTX-M-1 (20.6%), qnrB (20.6%), blaSHV (8.8%), aadB (5.9%), and qnrA (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in Campylobacter isolates was significantly (p ≤ 0.05) associated with the presence of blaTEM, tetA, and blaSHV genes, respectively. The high rates of AMR in Campylobacter isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived Campylobacter isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.
期刊介绍:
Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide.
Foodborne Pathogens and Disease coverage includes:
Agroterrorism
Safety of organically grown and genetically modified foods
Emerging pathogens
Emergence of drug resistance
Methods and technology for rapid and accurate detection
Strategies to destroy or control foodborne pathogens
Novel strategies for the prevention and control of plant and animal diseases that impact food safety
Biosecurity issues and the implications of new regulatory guidelines
Impact of changing lifestyles and consumer demands on food safety.