从孟加拉国两个地区的养鸡场中发现的弯曲杆菌属抗菌药耐药性概况。

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Foodborne pathogens and disease Pub Date : 2024-04-02 DOI:10.1089/fpd.2023.0079
Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam
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引用次数: 0

摘要

弯曲杆菌中抗菌素耐药性(AMR)的迅速出现,加强了其作为引起重大公共卫生问题的食源性病原体的地位。耐药性弯曲杆菌通常通过食用受污染的动物产品(尤其是家禽)传染给人类。人们对弯曲杆菌属抗菌药耐药性的相关基因知之甚少。为了填补这一知识空白,我们对孟加拉国两个地区的 84 个养鸡场进行了 AMR 弯曲状杆菌流行率调查。我们收集了鸡的泄殖腔拭子,并对弯曲杆菌进行了细菌学检测和 PCR 确认。采用磁盘扩散法检测了 14 种抗生素的抗菌药敏感性,并使用多重 PCR 筛选了弯曲杆菌阳性分离物中的 12 种耐药基因。共有 34 个(40.5%)农场的弯曲菌呈阳性,其中 73.5%的分离株对至少 10 种抗生素具有耐药性。抗菌药敏感性结果显示,对链霉素(97.1%)、林可霉素(97.1%)、氨苄西林(94.1%)、四环素(94.1%)、红霉素(91.2%)、环丙沙星(88.2%)、萘啶酸(85.3%)和亚胺培南(82.4%),对氯霉素(47.1%)、头孢他啶(44.1%)和大肠杆菌素(35.3%)的耐药率相对较低。在97.1%和50%的分离株中分别发现了耐多药(MDR)和广泛耐药弯曲杆菌。共鉴定出 10 个耐药基因,包括 blaTEM(97.1% 的分离株)、strA-strB(85.9%)、tetA(70.6%)、tetB(32.4%)、qnrS(23.5%)、blaCTX-M-1(20.6%)、qnrB(20.6%)、blaSHV(8.8%)、aadB(5.9%)和 qnrA(2.9%)。我们的研究结果表明,弯曲菌分离株对氨苄西林、四环素和头孢他啶的耐药性分别与 blaTEM、tetA 和 blaSHV 基因的存在有显著相关性(p ≤ 0.05)。鉴于农场中抗菌药物的自由使用和生物安全规定的不完善,我们研究中弯曲杆菌分离物的高 AMR 率并不令人惊讶。尤其令人担忧的是对人类专用抗生素(阿奇霉素、环丙沙星和可乐定)的耐药率。在使用多种抗生素、对禽类进行预防性治疗以及抗生素包装处理不当的养鸡场,AMR 的发生率更高。在我们的研究中,来自不同地区的鸡源弯曲杆菌分离物中 MDR 的流行率很高,这说明孟加拉国养鸡场需要更加谨慎地使用抗菌化合物。
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Antimicrobial Resistance Profiles of Campylobacter spp. Recovered from Chicken Farms in Two Districts of Bangladesh.

The rapid emergence of antimicrobial resistance (AMR) in Campylobacter has reinforced its status as a foodborne pathogen of significant public health concern. Resistant Campylobacter is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in Campylobacter spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR Campylobacter across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for Campylobacter spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in Campylobacter-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were Campylobacter-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant Campylobacter were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including blaTEM (in 97.1% of isolates), strA-strB (85.9%), tetA (70.6%), tetB (32.4%), qnrS (23.5%), blaCTX-M-1 (20.6%), qnrB (20.6%), blaSHV (8.8%), aadB (5.9%), and qnrA (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in Campylobacter isolates was significantly (p ≤ 0.05) associated with the presence of blaTEM, tetA, and blaSHV genes, respectively. The high rates of AMR in Campylobacter isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived Campylobacter isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.

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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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