Nienke H van Teijlingen, Marleen Y van Smoorenburg, Ramin Sarrami-Forooshani, Esther M Zijlstra-Willems, John L van Hamme, Hanneke Borgdorff, Janneke HHM van de Wijgert, Elisabeth van Leeuwen, Joris A M van der Post, Karin Strijbis, Carla M S Ribeiro, Teunis B H Geijtenbeek
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引用次数: 0
摘要
阴道微生物群失调对 HIV-1 的性传播构成严重威胁。在阴道菌群失调期间,普雷沃特氏菌大量存在,并与 HIV-1 易感性增强有关;然而,其潜在机制仍不清楚。在此,我们研究了阴道细菌对阴道 CD4+ T 细胞的 HIV-1 易感性的直接影响。值得注意的是,预先暴露于Prevotella timonensis会增强阴道T细胞对HIV-1的摄取,导致病毒融合增加和病毒生成增强。而预先暴露于抗逆转录病毒抑制剂则可消除由普雷沃氏菌增强的感染。因此,我们的研究表明,阴道微生物群直接影响粘膜 CD4+ T 细胞的易感性,强调了阴道菌群失调诊断和治疗的重要性。
Prevotella timonensis bacteria associated with vaginal dysbiosis enhance HIV-1 susceptibility of vaginal CD4+ T cells
Dysbiosis of the vaginal microbiome poses a serious risk for sexual HIV-1 transmission. Prevotella spp. are abundant during vaginal dysbiosis and associated with enhanced HIV-1 susceptibility; however, underlying mechanisms remain unclear. Here, we investigated the direct effect of vaginal bacteria on HIV-1 susceptibility of vaginal CD4+ T cells. Notably, pre-exposure to Prevotella timonensis enhanced HIV-1 uptake by vaginal T cells, leading to increased viral fusion and enhanced virus production. Pre-exposure to antiretroviral inhibitors abolished Prevotella timonensis-enhanced infection. Hence, our study shows that the vaginal microbiome directly affects mucosal CD4+ T cell susceptibility, emphasising importance of vaginal dysbiosis diagnosis and treatment.