用于抗病毒筛选的表达分泌型荧光素酶的重组森纳卡病毒 A 的构建和表征

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Journal of virological methods Pub Date : 2024-04-04 DOI:10.1016/j.jviromet.2024.114932

Hao Wang , Yongfang Mo , Wenbo Liu , Qijie He , Tongwei Ren , Kang Ouyang , Ying Chen , Weijian Huang , Zuzhang Wei

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引用次数: 0

摘要

猪病毒 A（SVA）是一种新发现的与猪水泡病和新生儿死亡有关的皮卡病毒。开发含有外源报告基因的 SVA 为病毒研究提供了有力的工具。在本研究中，我们成功构建了一种表达高斯荧光素酶（Gluc）的重组 SVA，称为 rSVA-Gluc。研究发现，rSVA-Gluc在BHK-21细胞中的生长动力学与亲本病毒相当，Gluc活性与病毒生长曲线一致。遗传分析表明，插入的报告蛋白基因至少可稳定遗传六代。我们评估了 rSVA-Gluc 在抗病毒药物筛选中的作用，结果显示它有可能成为抗 SVA 的有效工具。

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Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening

Senecavirus A (SVA) is a newly identified picornavirus associated with swine vesicular disease and neonatal mortality. The development of an SVA incorporating an exogenous reporter gene provides a powerful tool for viral research. In this study, we successfully constructed a recombinant SVA expressing Gaussia Luciferase (Gluc), termed rSVA-Gluc. The growth kinetics of rSVA-Gluc in BHK-21 cells were found to be comparable to those of the parental virus, and Gluc activity paralleled the virus growth curve. Genetic analysis revealed stable inheritance of the inserted reporter protein genes for at least six generations. We evaluated the utility of rSVA-Gluc in antiviral drug screening, and the results highlighted its potential as an effective tool for such purposes against SVA.

Data availability statement

The data that support the findings of this study are available on request from the corresponding author.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.