Banu Kandil , Nevin Kurtdede , Alev Gürol Bayraktaroglu
{"title":"大鼠卵巢发情周期、妊娠和哺乳期热休克蛋白(HSP27、HSP60、HSP70 和 HSP90)的免疫组织化学定位和表达","authors":"Banu Kandil , Nevin Kurtdede , Alev Gürol Bayraktaroglu","doi":"10.1016/j.acthis.2024.152157","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 3","pages":"Article 152157"},"PeriodicalIF":2.3000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical localization and expression of heat shock proteins (HSP27, HSP60, HSP70, and HSP90) during the oestrous cycle, pregnancy, and lactation in rat ovaries\",\"authors\":\"Banu Kandil , Nevin Kurtdede , Alev Gürol Bayraktaroglu\",\"doi\":\"10.1016/j.acthis.2024.152157\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.</p></div>\",\"PeriodicalId\":6961,\"journal\":{\"name\":\"Acta histochemica\",\"volume\":\"126 3\",\"pages\":\"Article 152157\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta histochemica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0065128124000254\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta histochemica","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0065128124000254","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Immunohistochemical localization and expression of heat shock proteins (HSP27, HSP60, HSP70, and HSP90) during the oestrous cycle, pregnancy, and lactation in rat ovaries
This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.
期刊介绍:
Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted