作为治疗牛皮癣外用制剂的龙胆紫素载体壳聚糖纳米粒子

Nanomaterials Pub Date : 2024-03-29 DOI:10.3390/nano14070610
Guohua Cheng, Xiaojie Zhang, Huiling Zhang, Zhixuan Feng, Jiaxiu Cai, Jingjing Li, Libo Du, Ke Liu
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引用次数: 0

摘要

银屑病是一种慢性炎症性皮肤病,由遗传因素、免疫因素、环境因素和心理因素等多种因素诱发,以表皮增厚、角质细胞过度增殖、分化异常和过度炎症反应为特征。传统的银屑病治疗方法仍然面临挑战,因为疗效有限,副作用明显,而且容易复发。相比之下,外用疗法因其非侵入性和自用性,为银屑病的治疗提供了有利的选择。在这项研究中,Gentiopicrin(Gen)被包裹在脂质体中形成纳米药物,然后壳聚糖被覆盖在纳米药物上形成纳米药物递送系统(CS@Gen),用作治疗银屑病的外用药物。结果表明,CS@Gen 可诱导银屑病角朊细胞凋亡并抑制其增殖和迁移。此外,涂抹 CS@Gen 乳膏可显著降低银屑病小鼠的表皮厚度,减少皮肤鳞屑,并改善其他相关机制。同时,制备的 CS@Gen 能明显降低 IL-17a、Cxcl2、S100a、Mki67 等相关炎症因子的表达水平,从而间接抑制角朊细胞的炎症反应。总之,本研究为治疗银屑病的抗炎和免疫调节药物递送系统提供了一种理想的载体。
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Gentiopicrin-Loaded Chitosan Nanoparticles as a Topical Agent for the Treatment of Psoriasis
Psoriasis, a chronic inflammatory skin disease induced by various factors, including genetic factors, immune factors, environmental factors, and psychological factors, is characterized by thickening of the epidermis, excessive proliferation of keratinocytes, abnormal differentiation, and an excessive inflammatory response. Traditional treatments for psoriasis still face challenges because of limited curative effects, notable side effects, and a tendency for recurrence. In contrast, topical therapy provides a favorable option for psoriasis treatment because of its noninvasive and self-administered method. In this study, gentiopicrin (Gen) is encapsulated in the liposomes to form a nanodrug, and then chitosan is covered on the nanodrug to assemble the nanodrug delivery system (CS@Gen), which is used as a topical agent for treating psoriasis. Then M5 (a mixture of five pro-inflammatory cytokines, i.e., IL-17A, IL-22, IL-1α, oncostatin M, and TNF-α)-induced HacaT cells and imiquimod-induced psoriasis mouse models are established, whose results show that CS@Gen induces apoptosis and inhibits the proliferation and cell migration of psoriasis keratinocytes. Additionally, the application of CS@Gen cream can significantly reduce epidermal thickness, diminish skin scaling, and improve other related mechanisms in mice affected by psoriasis. Meanwhile, the prepared CS@Gen can significantly reduce the expression levels of IL-17a, Cxcl2, S100a, Mki67, and other related inflammatory factors, resulting in indirectly inhibiting the inflammation of keratinocytes. In summary, the present study provides an ideal loading for an anti-inflammatory and immunomodulatory drug delivery system for the treatment of psoriasis.
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