探索小鼠体内 SARS-CoV 和 SARS-CoV-2 的交叉免疫性

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-03-26 DOI:10.2217/fvl-2023-0212

Shuhui Wang, Li Ren, Ying Liu, Xiuli Shen, Yanling Hao, Yiming Shao

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引用次数: 0

摘要

目的：本研究旨在开发一种通用疫苗，以有效应对未来冠状病毒感染的爆发。材料与方法：我们开发了针对 SARS-CoV 和 SARS-CoV-2 的重组 DNA 疫苗和疫苗载体疫苗，并在 BALB/c 小鼠中进行了评估。对细胞和体液免疫反应进行了分析。结果显示与单独免疫 SARS-CoV 或 SARS-CoV-2 疫苗的小鼠相比，交叉免疫组的细胞免疫反应增加了 21.2 倍，结合抗体增加了 80 多倍，中和抗体增加了 136.7 倍。与单独接种 DNA 疫苗相比，联合接种重组 DNA 和疫苗可诱导更强的免疫反应。结论这项研究表明，不同冠状病毒的交叉免疫可诱导广泛的免疫反应。

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Exploration of the cross-immunity between SARS-CoV and SARS-CoV-2 in mice

Aim: This study aimed to develop a universal vaccine that can effectively address future outbreaks of coronavirus infections. Materials & methods: We developed recombinant DNA vaccines and vaccinia vector vaccines specific for SARS-CoV and SARS-CoV-2 and evaluated them in BALB/c mice. Both cellular and humoral immune responses were analyzed. Results: Compared with those immunized with the SARS-CoV or SARS-CoV-2 vaccine alone, the cross-immune group exhibited a 21.2-fold increase in cellular immune response, an over 80-fold increase in binding antibodies and a 136.7-fold increase in neutralizing antibodies. The combination of recombinant DNA and vaccinia vaccines induced a stronger immune response than DNA vaccine immunization alone. Conclusion: This study demonstrated that cross-immunization with different coronaviruses can induce a broad immune response.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.