{"title":"褐藻粉、小球藻粉、梭状芽孢杆菌蛋白和棉籽蛋白浓缩物混合物替代鱼粉对吉贝鲤（Carassius gibelio, CAS Ⅴ）生长、蛋白质利用和肠道健康的影响","authors":"Yongning Yu, Xing Wang, Junyan Jin, Dong Han, Xiaoming Zhu, Haokun Liu, Zhimin Zhang, Yunxia Yang, Shouqi Xie","doi":"10.1155/2024/5019899","DOIUrl":null,"url":null,"abstract":"<p>The trial was conducted to investigate the effects of the replacement of dietary fishmeal (FM) by the blend of <i>Tenebrio molitor</i> meal (TMM), <i>Chlorella</i> meal (CM), <i>Clostridium autoethanogenum</i> protein (CAP), cottonseed protein concentrate (CPC) on growth, protein utilization and intestinal health of gibel carp (<i>Carassius gibelio</i>, CAS Ⅴ). The FM-based diet was used as the control, and the blended proteins (TMM: CM: CAP: CPC) at ratios of 1 : 1:8 : 2 (BLEND A), 1 : 1:6 : 4 (BLEND B), and 1 : 1:4 : 6 (BLEND C) were used to replace FM at three levels (33%, 67%, 100%), respectively. The results showed that, compared to the control group, growth performance increased significantly when dietary FM was fully replaced by BLEND B (<i>P</i> < 0.05), while decreased by BLEND A (<i>P</i> < 0.05). The complete substitution of FM with BLEND B significantly upregulated the mRNA expression of intestinal proinflammatory cytokines, anti-inflammatory cytokines, and tight junction-related genes (<i>P</i> < 0.05), improving intestinal tissue morphology and health. And it also significantly increased intestinal trypsin activity (<i>P</i> < 0.05), upregulated the mRNA expression of amino acid sensory receptor-related and amino acid or peptide transport-related genes (<i>P</i> < 0.05), increased protein apparent digestibility coefficient (<i>P</i> < 0.05). The 100% substitution of FM with BLEND A significantly upregulated the mRNA expression of intestinal proinflammatory cytokines and downregulated the mRNA expression of anti-inflammatory cytokine <i>il-10</i> (<i>P</i> < 0.05), reduced intestinal villus height (<i>P</i> < 0.05), and decreased protein apparent digestibility coefficient (<i>P</i> < 0.05). In conclusion, BLEND B could completely substitute dietary FM and was beneficial to the growth and health of gibel carp. Dietary digestible essential amino acids index (DEEAI) was found as an important indicator and should be higher than 79.5% to meet the maximum growth of fish.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of the Replacement of Dietary Fishmeal by the Blend of Tenebrio molitor Meal, Chlorella Meal, Clostridium Autoethanogenum Protein, and Cottonseed Protein Concentrate on Growth, Protein Utilization, and Intestinal Health of Gibel Carp (Carassius gibelio, CAS Ⅴ)\",\"authors\":\"Yongning Yu, Xing Wang, Junyan Jin, Dong Han, Xiaoming Zhu, Haokun Liu, Zhimin Zhang, Yunxia Yang, Shouqi Xie\",\"doi\":\"10.1155/2024/5019899\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The trial was conducted to investigate the effects of the replacement of dietary fishmeal (FM) by the blend of <i>Tenebrio molitor</i> meal (TMM), <i>Chlorella</i> meal (CM), <i>Clostridium autoethanogenum</i> protein (CAP), cottonseed protein concentrate (CPC) on growth, protein utilization and intestinal health of gibel carp (<i>Carassius gibelio</i>, CAS Ⅴ). The FM-based diet was used as the control, and the blended proteins (TMM: CM: CAP: CPC) at ratios of 1 : 1:8 : 2 (BLEND A), 1 : 1:6 : 4 (BLEND B), and 1 : 1:4 : 6 (BLEND C) were used to replace FM at three levels (33%, 67%, 100%), respectively. The results showed that, compared to the control group, growth performance increased significantly when dietary FM was fully replaced by BLEND B (<i>P</i> < 0.05), while decreased by BLEND A (<i>P</i> < 0.05). The complete substitution of FM with BLEND B significantly upregulated the mRNA expression of intestinal proinflammatory cytokines, anti-inflammatory cytokines, and tight junction-related genes (<i>P</i> < 0.05), improving intestinal tissue morphology and health. And it also significantly increased intestinal trypsin activity (<i>P</i> < 0.05), upregulated the mRNA expression of amino acid sensory receptor-related and amino acid or peptide transport-related genes (<i>P</i> < 0.05), increased protein apparent digestibility coefficient (<i>P</i> < 0.05). The 100% substitution of FM with BLEND A significantly upregulated the mRNA expression of intestinal proinflammatory cytokines and downregulated the mRNA expression of anti-inflammatory cytokine <i>il-10</i> (<i>P</i> < 0.05), reduced intestinal villus height (<i>P</i> < 0.05), and decreased protein apparent digestibility coefficient (<i>P</i> < 0.05). In conclusion, BLEND B could completely substitute dietary FM and was beneficial to the growth and health of gibel carp. Dietary digestible essential amino acids index (DEEAI) was found as an important indicator and should be higher than 79.5% to meet the maximum growth of fish.</p>\",\"PeriodicalId\":8225,\"journal\":{\"name\":\"Aquaculture Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-03-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/2024/5019899\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/5019899","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
摘要
本试验旨在研究用褐藻粉（TMM）、小球藻粉（CM）、自乙醇梭菌蛋白（CAP）和棉籽浓缩蛋白（CPC）的混合蛋白替代日粮鱼粉（FM）对草鱼（Carassius gibelio，CAS Ⅴ）的生长、蛋白质利用和肠道健康的影响。以 FM 日粮为对照，混合蛋白质（TMM: CM: CAP: CPC）的比例为 1 ：8 : 2（混合 a）、1 ：1:6 :4（混合 B）和 1 ：1 : 1 : 4 : 6（混合物 C），分别以 33%、67%、100% 三种比例替代调频。结果表明，与对照组相比，当日粮中的 FM 被 BLEND B 完全替代时，生长性能显著提高（P<0.05），而当日粮中的 FM 被 BLEND A 完全替代时，生长性能显著下降（P<0.05）。用BLEND B完全替代FM能显著提高肠道促炎细胞因子、抗炎细胞因子和紧密连接相关基因的mRNA表达（P<0.05），改善肠道组织形态和健康状况。它还能明显提高肠道胰蛋白酶活性（P<0.05），上调氨基酸感觉受体相关基因和氨基酸或肽转运相关基因的 mRNA 表达（P<0.05），提高蛋白质表观消化系数（P<0.05）。BLEND A 100%替代FM可显著上调肠道促炎细胞因子的mRNA表达，下调抗炎细胞因子il-10的mRNA表达（P<0.05），降低肠绒毛高度（P<0.05），降低蛋白质表观消化系数（P<0.05）。总之，BLEND B 可完全替代日粮中的调频，对长臂猿的生长和健康有益。膳食可消化必需氨基酸指数（DEEAI）是一项重要指标，应高于 79.5%才能满足鱼类的最大生长需求。
Effects of the Replacement of Dietary Fishmeal by the Blend of Tenebrio molitor Meal, Chlorella Meal, Clostridium Autoethanogenum Protein, and Cottonseed Protein Concentrate on Growth, Protein Utilization, and Intestinal Health of Gibel Carp (Carassius gibelio, CAS Ⅴ)
The trial was conducted to investigate the effects of the replacement of dietary fishmeal (FM) by the blend of Tenebrio molitor meal (TMM), Chlorella meal (CM), Clostridium autoethanogenum protein (CAP), cottonseed protein concentrate (CPC) on growth, protein utilization and intestinal health of gibel carp (Carassius gibelio, CAS Ⅴ). The FM-based diet was used as the control, and the blended proteins (TMM: CM: CAP: CPC) at ratios of 1 : 1:8 : 2 (BLEND A), 1 : 1:6 : 4 (BLEND B), and 1 : 1:4 : 6 (BLEND C) were used to replace FM at three levels (33%, 67%, 100%), respectively. The results showed that, compared to the control group, growth performance increased significantly when dietary FM was fully replaced by BLEND B (P < 0.05), while decreased by BLEND A (P < 0.05). The complete substitution of FM with BLEND B significantly upregulated the mRNA expression of intestinal proinflammatory cytokines, anti-inflammatory cytokines, and tight junction-related genes (P < 0.05), improving intestinal tissue morphology and health. And it also significantly increased intestinal trypsin activity (P < 0.05), upregulated the mRNA expression of amino acid sensory receptor-related and amino acid or peptide transport-related genes (P < 0.05), increased protein apparent digestibility coefficient (P < 0.05). The 100% substitution of FM with BLEND A significantly upregulated the mRNA expression of intestinal proinflammatory cytokines and downregulated the mRNA expression of anti-inflammatory cytokine il-10 (P < 0.05), reduced intestinal villus height (P < 0.05), and decreased protein apparent digestibility coefficient (P < 0.05). In conclusion, BLEND B could completely substitute dietary FM and was beneficial to the growth and health of gibel carp. Dietary digestible essential amino acids index (DEEAI) was found as an important indicator and should be higher than 79.5% to meet the maximum growth of fish.
期刊介绍：
Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers.
Aquaculture Nutrition publishes papers which strive to:
increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research.
improve understanding of the relationships between nutrition and the environmental impact of aquaculture.
increase understanding of the relationships between nutrition and processing, product quality, and the consumer.
help aquaculturalists improve their management and understanding of the complex discipline of nutrition.
help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.
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