N. Sahu, Devarchan Nirala, Anil Kumar Singh, J. K. Tiwari
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引用次数: 0
摘要
本研究利用 29 个 SSR（微卫星）标记和形态特征研究了 100 个蚕豆种质品系和三个对照品系的种群结构和遗传多样性。ET218734、ET226572 和 ET218734 基因型表现出最高的种子产量和较高的各种产量性状平均值。D2 统计分析将基因型分为五个群。SSR 标记分析显示，在所研究的基因型中，每个位点平均有 2 个等位基因，从 2 个到 3 个不等。这些标记的多态信息含量（PIC）从 0.063 到 0.492 不等，平均为 0.37。种群结构分析将所研究的基因型分为三大类。分子多样性距离在簇 III 和簇 I 中最大（0.289），而在簇 II 和簇 I 中最小（0.148）。等位基因频率差异（预期杂合度）在簇 II 中最大（0.164），在簇 III 中最小（0.096）。
Population structure and genetic diversity analysis in faba bean (Vicia faba L.) germplasm using SSR markers
In the present study, population structure and genetic diversity were studied among 100 faba bean germplasm lines along with three checks using 29 SSR (microsatellite) markers and morphological traits. ET218734, ET226572, and ET218734 genotypes exhibited maximum seed yield and high mean performance for various yield-attributing traits. The D2 statistics analysis grouped genotypes into five clusters. SSR marker analysis revealed, on average, 2 alleles per locus ranging from 2 to 3 among the studied genotypes. Polymorphic information content (PIC) for these markers ranged from 0.063 to 0.492 with an average of 0.37. Population structure analysis classified the studied genotypes into three major clusters. The Molecular Diversity Distance was found to be greatest among Cluster III and Cluster I (0.289), while the lowest was among Cluster II and Cluster I (0.148). The allele frequency divergence (expected heterozygosity) was found highest on Cluster II (0.164), and the lowest was on Cluster III (0.096).
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