胎儿时期人类椎间盘结构转变的动态变化

L. V. Vikhareva, Viktoriya V. Makarova
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引用次数: 0

摘要

背景:人们对椎间盘结构的形成过程仍然知之甚少。因此,对作为椎间盘强度和弹性基础的纤维成分在出生后的发育预测进行评估似乎是合适的。目的:确定纤维环和髓核在胎儿期的微观结构转变,并将其与脊柱运动节段的本体定向进行比较。材料与方法:研究材料包括在对 50 个胎儿进行尸检时获得的 150 个椎间盘。其中 42 名胎儿的胎龄在胎儿早期,8 名胎儿的胎龄在胎儿晚期。对每个胎儿的椎间盘СV-СVI、ThV-ThVI、LV-SI进行了检查。所有组织学制备均采用苏木精和伊红、银浸渍法、PAS 反应、阿尔西安蓝(pH=1.0)、Van Gieson 和 Weigert 染色。组间比较采用 Kruskal-Wallis、Mann-Whitney 和 Newman-Keuls 检验。P≤0.05为差异有统计学意义。结果:胎儿晚期的弹性纤维出现在纤维环和髓核外周区。胶原纤维和弹性纤维的染色强度在纤维环外层更为明显。对血管连接组织形态参数的分析表明,其数量增加(p=0.0368),椎间盘血管连接组织形态中的血管数量增加(p=0.0449),其方向是脊柱运动节段的低层。这两项指标在СV-СVI和LV-SIdiscs之间以及СV-СVI和ThV-ThVIdiscs之间存在差异。在所有研究的颈椎和胸椎血管结缔组织形态参数中,胎儿早期和晚期的差异无统计学意义。结论:椎间盘的发育是由外周向中心进行的;其进一步发育的源头是纤维环。
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Dynamics of structural transformations of intervertebral discs in humans in the fetal period
BACKGROUND:The process of formation of the intervertebral disc structure remains poorly understood. Therefore, evaluating the forecasts for the development of the disc’s fibrous component as the foundation of its strength and elastic properties in the postnatal period seems appropriate. AIM:To identify microstructural transformations of the annulus fibrosus and nucleus pulposus in the fetal period and compare them with the ontogenetic orientation of the spinal motion segment. MATERIAL AND METHODS:The study material included 150intervertebral discs obtained during autopsy of 50fetuses. The gestational age of 42fetuses was in the early fetal period, 8fetuses were in the late fetal period. Intervertebral discs СV–СVI, ThV–ThVI, LV–SIwere examined in each fetus. All histological preparations were stained with hematoxylin and eosin, silver impregnation method, PAS reaction, Alcian blue (pH=1.0), Van Gieson and Weigert stains. For intergroup comparisons, the Kruskal–Wallis, Mann–Whitney, and Newman–Keuls tests were used. Differences were considered statistically significant atp≤0.05. RESULTS:Elastic fibers in the late fetal period were found in the annulus fibrosus and the peripheral zone of the nucleus pulposus. The intensity of staining of collagen and elastic fibers was more pronounced in the outer layers of the annulus fibrosus. Analysis of the parameters of the vascular-connective tissue formations showed an increase in their number (p=0.0368) and an increase in the number of vessels in the vascular-connective tissue formations of the intervertebral disc (p=0.0449) in the direction of the lower levels of the spinal motion segments. Differences in these two indicators were obtained between the СV–СVIand LV–SIdiscs, as well as between the СV–СVIand ThV–ThVIdiscs. In relation to all the studied morphometric parameters of the vascular-connective tissue formations in the cervical and thoracic spine, no statistically significant differences were obtained between the early and late fetal periods. CONCLUSION:The development of the intervertebral disc occurs from the peripheral parts to the center; the source for its further development is the fibrous ring.
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