S. Peerakietkhajorn, Wirawan Worakit, C. Moukamnerd, C. Tipbunjong
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引用次数: 0
摘要
蓝藻是一种海洋藻类,在亚洲和大洋洲被广泛养殖和食用。目前,新兴的绿色技术在植物化学提取方面很受欢迎。因此,本研究旨在采用高压锅辅助萃取法和微波辅助萃取法(分别为 A-CLE 和 M-CLE),研究蓝藻提取物的近似成分、植物化学成分和抗氧化特性。测试了提取物对人体正常结肠细胞和淡水甲壳类水蚤的毒性。我们发现,A-CLE 中的粗脂肪、灰分和水分含量低于 M-CLE,而粗蛋白和粗碳水化合物含量高于 A-CLE。A-CLE的总黄酮含量(1.474 ± 0.046 mg QE g-1提取物)高于M-CLE(1.179 ± 0.054 mg QE g-1提取物)。两种提取物的总酚含量、三萜含量和抗氧化活性没有差异。A-CLE 的 IC50(12,260 ± 197 µg mL-1)高于 M-CLE 的 IC50(10,950 ± 169 µg mL-1)。在急性毒性试验中,A-CLE 对 D. magna 的半数致死浓度(7.50 ± 0.28 g L-1)低于 M-CLE 的半数致死浓度(8.76 ± 0.26 g L-1)。这项研究表明,高压锅辅助萃取和微波辅助萃取是萃取 C. lentillifera 的有效绿色方法。这项研究将有助于对扁柏提取物进行进一步研究,以改善人类和动物的健康状况。
Proximate Composition, Phytochemical Analysis and Toxicity Assessment of Extracts of Caulerpa lentillifera using Autoclave- and Microwave-Assisted Extractions
Caulerpa lentilliferais a marine alga widely cultured and consumed in Asia and Oceania. Emerging green techniques are currently popular for phytochemical extraction. Therefore, this study aimed to investigate the proximate compositions, phytochemicals, and antioxidant properties of the C. lentilliferaextracts using autoclave-assisted and microwave-assisted extraction methods (A-CLE and M-CLE, respectively). The toxicity of extracts was tested against human normal colon cells and freshwater crustacean Daphnia magna. We found that crude lipid, ash and moisture contents in A-CLE were lower than those in M-CLE, while crude protein and crude carbohydrate were higher in A-CLE. Total flavonoid content in A-CLE (1.474 ± 0.046 mg QE g-1 extract) was higher than that in M-CLE (1.179 ± 0.054 mg QE g-1 extract). There was no difference in total phenolic contents, triterpenoid contents and antioxidant activities between the extracts. The IC50 of A-CLE (12,260 ± 197 µg mL-1) was higher than that of M-CLE (10,950 ± 169 µg mL-1). In the acute toxicity test, the LC50 of A-CLE against D. magna (7.50 ± 0.28 g L-1) was lower than LC50 of M-CLE (8.76 ± 0.26 g L-1). This study suggest that autoclave-assisted and microwave-assisted extractions are effective green methods of extracting C. lentillifera. This study will be useful for further studies of C. lentillifera extracts to improve human and animal health.
期刊介绍:
Sains Malaysiana is a refereed journal committed to the advancement of scholarly knowledge and research findings of the several branches of science and technology. It contains articles on Earth Sciences, Health Sciences, Life Sciences, Mathematical Sciences and Physical Sciences. The journal publishes articles, reviews, and research notes whose content and approach are of interest to a wide range of scholars. Sains Malaysiana is published by the UKM Press an its autonomous Editorial Board are drawn from the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. In addition, distinguished scholars from local and foreign universities are appointed to serve as advisory board members and referees.