Seyedeh Zahra Parkhideh, Mehdi Forouzesh, Maryam Akhgari, L. Bahmanabadi, F. Jokar, Samira Valiyari, K. Akbarzadeh, Sara Rahimi, Ali Mohammad Alimohammadi, S. Rafizadeh, Y. Rassi
{"title":"在甲基苯丙胺处理过的肉类中饲养的蚕蛾(双翅目:茧蜂科)不同发育阶段甲基苯丙胺检测的初步分析","authors":"Seyedeh Zahra Parkhideh, Mehdi Forouzesh, Maryam Akhgari, L. Bahmanabadi, F. Jokar, Samira Valiyari, K. Akbarzadeh, Sara Rahimi, Ali Mohammad Alimohammadi, S. Rafizadeh, Y. Rassi","doi":"10.18502/jad.v17i3.14984","DOIUrl":null,"url":null,"abstract":"Background: It is possible to identify drugs and poisons present in cadavers by analyzing blowfly larvae and pupae collected during forensic autopsies. The main purpose of this study was to use Lucilia sericata’s larvae and pupae to identify drugs and poisons present in human cadavers. \nMethods: In an investigation, immature L. sericata fed meat treated with methamphetamine (MA) at various concentrations (45, 90, and 180 ng/mg) were analyzed to detect MA. Acetylation derivatization and liquid-liquid extraction (LLE) were used as sample preparation methods prior to gas chromatography-mass spectrometry (GC-MS) analytical instrumentation to find MA. \nResults: According to this study, L. sericata can be used in toxicological testing to identify MA in a host body. All L. sericata larval stages, particularly the third stage larva, pupa and empty pupa tested were positive for MA. Larvae in their first instar produced weak peaks. The L. sericata post-feeding instar following the 45 ng/mg treatment showed the highest MA concentration. For the first time, derivatization using the acetylation approach was used to prepare samples, and successfully, excellent results were obtained. \nConclusion: Low quantities of MA can be easily found in immature fly samples using GC-MS. It is important to analyze all samples including human tissues and insect samples, for postmortem drug testing. They can be utilized to find entire MA before they are excreted in excretory samples such as urine. Also, third instar larvae are a great and reliable sample for toxicological study.","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preliminary Analysis of Methamphetamine Detection in Lucilia sericata (Diptera: Calliphoridae) Reared in Methamphetamine-Treated Meat at Various Developmental Stages\",\"authors\":\"Seyedeh Zahra Parkhideh, Mehdi Forouzesh, Maryam Akhgari, L. Bahmanabadi, F. Jokar, Samira Valiyari, K. Akbarzadeh, Sara Rahimi, Ali Mohammad Alimohammadi, S. Rafizadeh, Y. Rassi\",\"doi\":\"10.18502/jad.v17i3.14984\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: It is possible to identify drugs and poisons present in cadavers by analyzing blowfly larvae and pupae collected during forensic autopsies. The main purpose of this study was to use Lucilia sericata’s larvae and pupae to identify drugs and poisons present in human cadavers. \\nMethods: In an investigation, immature L. sericata fed meat treated with methamphetamine (MA) at various concentrations (45, 90, and 180 ng/mg) were analyzed to detect MA. Acetylation derivatization and liquid-liquid extraction (LLE) were used as sample preparation methods prior to gas chromatography-mass spectrometry (GC-MS) analytical instrumentation to find MA. \\nResults: According to this study, L. sericata can be used in toxicological testing to identify MA in a host body. All L. sericata larval stages, particularly the third stage larva, pupa and empty pupa tested were positive for MA. Larvae in their first instar produced weak peaks. The L. sericata post-feeding instar following the 45 ng/mg treatment showed the highest MA concentration. For the first time, derivatization using the acetylation approach was used to prepare samples, and successfully, excellent results were obtained. \\nConclusion: Low quantities of MA can be easily found in immature fly samples using GC-MS. It is important to analyze all samples including human tissues and insect samples, for postmortem drug testing. They can be utilized to find entire MA before they are excreted in excretory samples such as urine. 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引用次数: 0
摘要
背景:通过分析法医解剖尸体时收集到的蝇类幼虫和蛹,可以识别尸体中的毒品和毒药。本研究的主要目的是利用绢毛琉璃苣(Lucilia sericata)的幼虫和蛹来鉴定人类尸体中的药物和毒物。方法:在一项调查中,分析了用不同浓度(45、90 和 180 纳克/毫克)的甲基苯丙胺(MA)处理过的蚕蛹肉,以检测 MA。在使用气相色谱-质谱(GC-MS)分析仪器检测甲基苯丙胺之前,采用乙酰化衍生和液液萃取(LLE)作为样品制备方法。研究结果根据这项研究,血清矛尾蚴(L. sericata)可用于毒理学测试,以鉴定宿主体内的 MA。所有 L. sericata 幼虫阶段,尤其是第三阶段幼虫、蛹和空蛹的 MA 检测结果均呈阳性。第一龄幼虫产生的峰值较弱。经 45 毫微克/毫克处理后的蚕食后幼虫显示出最高的 MA 浓度。首次使用乙酰化方法衍生制备样品,并成功地获得了极佳的结果。结论使用气相色谱-质谱法很容易在未成熟的苍蝇样品中发现低浓度的 MA。分析包括人体组织和昆虫样本在内的所有样本对死后药物检测非常重要。在 MA 从尿液等排泄物样本中排出之前,可以利用它们来发现全部 MA。此外,三龄幼虫也是进行毒理学研究的可靠样本。
Preliminary Analysis of Methamphetamine Detection in Lucilia sericata (Diptera: Calliphoridae) Reared in Methamphetamine-Treated Meat at Various Developmental Stages
Background: It is possible to identify drugs and poisons present in cadavers by analyzing blowfly larvae and pupae collected during forensic autopsies. The main purpose of this study was to use Lucilia sericata’s larvae and pupae to identify drugs and poisons present in human cadavers.
Methods: In an investigation, immature L. sericata fed meat treated with methamphetamine (MA) at various concentrations (45, 90, and 180 ng/mg) were analyzed to detect MA. Acetylation derivatization and liquid-liquid extraction (LLE) were used as sample preparation methods prior to gas chromatography-mass spectrometry (GC-MS) analytical instrumentation to find MA.
Results: According to this study, L. sericata can be used in toxicological testing to identify MA in a host body. All L. sericata larval stages, particularly the third stage larva, pupa and empty pupa tested were positive for MA. Larvae in their first instar produced weak peaks. The L. sericata post-feeding instar following the 45 ng/mg treatment showed the highest MA concentration. For the first time, derivatization using the acetylation approach was used to prepare samples, and successfully, excellent results were obtained.
Conclusion: Low quantities of MA can be easily found in immature fly samples using GC-MS. It is important to analyze all samples including human tissues and insect samples, for postmortem drug testing. They can be utilized to find entire MA before they are excreted in excretory samples such as urine. Also, third instar larvae are a great and reliable sample for toxicological study.
期刊介绍:
The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including:
● Systematics
● Vector ecology
● Epidemiology
● Immunology
● Parasitology
● Molecular biology
● Genetics
● Population dynamics
● Toxicology
● Vector control
● Diagnosis and treatment and other related subjects.