在两亲化合物的保护下,对受到后高渗休克影响的人类红细胞中磷脂酰丝氨酸的形态和分布进行评估

O. Nipot, N. Yershova, O. Chabanenko, P.M. Zubov, N. Shpakova
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引用次数: 0

摘要

寻找可在红细胞解冻过程中使用的保护物质并研究其对红细胞的影响,有助于提高低温保存周期后存活细胞的数量和质量。我们研究了高渗后休克和两性化合物对人类红细胞形状和红细胞凋亡的影响。我们使用了流式细胞仪方法,这种方法可以同时分析两个参数,从而提高了研究效率。形状通过球形度指数(SphI)进行评估,红细胞凋亡通过磷脂酰丝氨酸在膜外表面的重新分布进行评估。研究表明,癸基硫酸钠和氯丙嗪可将高渗休克后的红细胞损伤分别减少 3.6 倍和 4.2 倍。癸基硫酸钠有助于保持细胞形状(SphI 系数保持不变),而使用氯丙嗪时,细胞形状会向球形转变(SphI 系数变化 2 倍)。对膜外层磷脂酰丝氨酸与 Annexin V FITC 结合水平的研究表明,当使用癸基硫酸钠时,荧光的增加与浓度有关,这表明双分子层的不对称性发生了紊乱。相反,氯丙嗪不会改变磷脂酰丝氨酸的分布。通过对细胞活力的两个参数--球形系数和anexin结合力--进行比较,我们选择了使用所研究的保护物质的最佳条件。也就是说,最好使用最低有效浓度的癸基硫酸钠(200 mcmol/l)进行保护。这样既能保持细胞形状,又能将对膜不对称性的影响降至最低。
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EVALUATION OF THE FORM AND DISTRIBUTION OF PHOSPHATIDYLSERINE IN HUMAN ERYTHROCYTES EXPOSED TO POSTHYPERTONIC SHOCK UNDER THE PROTECTION OF AMPHIPHILIC COMPOUNDS
The search for protective substances that can be used during red blood cell thawing and the study of their effects on red blood cells contribute to increasing the number and quality of viable cells after the cryopreservation cycle. We studied the effect of posthypertonic shock and amphiphilic compounds on the shape and eryptosis of human erythrocytes. The method of flow cytometry was used, this allows analyzing two parameters simultaneously, which increases the efficiency of research. The shape was assessed by the sphericity index (SphI), and eryptosis by the redistribution of phosphatidylserine to the membrane outer surface. It has been shown that sodium decylsulfate and chlorpromazine reduce erythrocyte damage in posthypertonic shock by 3.6 and 4.2 times, respectively. Sodium decylsulfate helps to preserve the shape of cells (SphI coefficient remains the same), while when chlorpromazine is used, the shape changes towards spherical (SphI coefficient changes 2 times). The study of the level of Annexin V FITC binding to phosphatidylserine in outer layer of membrane revealed a concentration-dependent increase in fluorescence when sodium decylsulfate was used, indicating a disorder of the bilayer asymmetry. In contrast, chlorpromazine did not change the distribution of phosphatidylserine. Comparison of two parameters of cell viability - the sphericity coefficient and anexin binding - allowed us to choose the conditions that are optimal for the use of the studied protective substances. Namely, it is advisable to use the lowest effective concentration of sodium decylsulfate (200 mcmol/l) for protective purposes. This ensures the preservation of the cell shape and minimal impact on the membrane asymmetry.
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EVALUATION OF THE FORM AND DISTRIBUTION OF PHOSPHATIDYLSERINE IN HUMAN ERYTHROCYTES EXPOSED TO POSTHYPERTONIC SHOCK UNDER THE PROTECTION OF AMPHIPHILIC COMPOUNDS Evaluation of the component’s contribution in endothelium-dependent acetylcholine-induced relaxation of the rat aorta L-tryptophan effectively prevents fatty degeneration of rat pancreas ROLE OF POTASSIUM CHANNEL TREK-1 IN MECHANOSENSITIVITY OF SMOOTH MUSCLE CELLS FROM RAT DETRUSOR The influence of phenformin on the extracellular matrix of the liver of rats under long-term administration of ethanol [
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