用气液相色谱-质谱检测法比较分析胸腺蛇床子和胸腺马钱子精油的化学成分精油化学成分的比较分析

A. Sheremetyeva, L. V. Karavaeva, N. Durnova, O. Shapoval, N. Mukhamadiev, G. Rabbimova, M. H. Nazirbekov
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摘要

引言植物精油是不同化合物的天然来源,它们对大型生物的作用取决于其化学成分,取决于环境因素,包括植物种类。研究目的本研究旨在比较从 Thymus serpyllum L. 和 Thymus marschallianus Willd.(唇形科植物)气生部分提取的精油的化学成分。材料和方法百里香(Thymus marschallianus)的植物原料是六月在萨拉托夫州的开花期采集的,百里香(Thymus serpyllum)的植物原料是在药房网络购买的。精油是通过金斯伯格水蒸气蒸馏法获得的。化学成分分析采用气液色谱法和质谱检测法进行。结果显示两种精油的主要成分都是百里酚及其异构体,它们在 T. marschallianus 精油中的质量分数为 38.4%,在 T. serpyllum 精油中的质量分数为 44%。比较分析采用非参数 Mann-Whitney 标准。三环倍半萜烯的总含量在 T. marschallianus 精油中为 4.73%,在 T. serpyllum 精油中为 3.59%。结论。根据化学成分,上述植物物种的精油属于酚类化学类型,主要化合物相似。
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COMPARATIVE ANALYSIS OF CHEMICAL COMPOSITION OF THYMUS SERPYLLUM L. AND THYMUS MARSCHALLIANUS WILLD. ESSENTIAL OILS BY GAS-LIQUID CHROMATOGRAPHY WITH MASS SPECTROMETRY DETECTION
Introduction. Plant essential oils are natural sources of the different compounds, the action of which in the macroorganism is determined by their chemical composition, depending on environmental factors, including plant species. Aim. The aim of the study was to compare the chemical composition of essential oils, obtained from the aerial parts of Thymus serpyllum L. and Thymus marschallianus Willd. (Lamiaceae). Material and methods. The plant raw materials of Thymus marschallianus were collected in the flowering phase in the Saratov region in June, the plant raw materials of Thymus serpyllum were purchased in the pharmacy network. The essential oils were obtained by Ginsberg’s steam hydrodistillation method. Analysis of their chemical composition was performed by gas-liquid chromatography with mass spectrometry detection. Results. The main components of both types of the essential oils were represented by thymol and its isomers, their mass fraction in the essential oil of T. marschallianus is 38.4, in the essential oil of T. serpyllum is 44%. The comparative analysis was carried out using the non-parametric Mann-Whitney criterion. Statistically significant differences with 95% probability were established for tricyclic sesquiterpene alkenes, the total content of which in the essential oil of T. marschallianus is 4.73%, in the essential oil of T. serpyllum – 3.59%. Conclusion. According to their chemical composition the essential oils of the mentioned plant species belong to phenolic chemotype and are similar in the major compounds.
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