筛选作为 NPV 日光保护剂的佐剂,并评估针对斑翅虫的储存条件

Navdisha, Neelam Joshi, Sudhendu Sharma
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引用次数: 0

摘要

这项实验研究评估了在阳光下补充佐剂对核多角体病病毒幼虫死亡率的影响。研究表明,1%的锡诺帕对 SpltNPV(原生病毒)和 SpltNPV(NIPHM)对斑翅蝶(雌性)的三龄幼虫提供了最好的日光保护,病毒效力分别高达 68.75% 和 66.75%。然而,与对照组(单独使用病毒)相比,所有辅助剂在阳光下都能显著提高幼虫死亡率。在不同储存条件下储存的 SpltNPV 病毒制剂,在冷藏温度(4± 2°C)下储存在琥珀色玻璃瓶中时,幼虫死亡率的降低幅度最小。
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Screening of Adjuvants as Sunlight Protectant for NPV and Evaluation of Storage Conditions against Spodoptera litura
This experimental study evaluated the effect of adjuvant supplementation on larval mortality of nuclear polyhedrosis virus under sunlight. Study revealed that Tinopal @1% provided best protection from sunlight and retained viral efficacy up to 68.75 and 66.75% in SpltNPV (native) and SpltNPV (NIPHM) respectively, against third instar larvae of Spodoptera litura (F). However, all adjutants resulted in significantly higher larval mortality as compared to control (virus alone) under sunlight. SpltNPV viral formulations stored at different storage conditions recorded minimum reduction in larval mortality, when stored in amber coloured glass bottle at refrigeration temperature (4± 2°C).
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