Paula A. Ossa-lópez, J. Uribe, H. Ramírez-chaves, F. A. Rivera-Páez
{"title":"Amblyomma triste Koch, 1844 (Acari: Ixodidae) 的首个线粒体基因组:A. maculatum 组内研究物种的证据","authors":"Paula A. Ossa-lópez, J. Uribe, H. Ramírez-chaves, F. A. Rivera-Páez","doi":"10.11158/saa.29.1.7","DOIUrl":null,"url":null,"abstract":"The tick species that comprise the Amblyomma maculatum group (Acari: Ixodidae) are widely distributed in the Nearctic and Neotropical regions, ranging from the United States of America (USA) to Argentina. This group includes three species: Amblyomma maculatum, Amblyomma tigrinum, and Amblyomma triste, which parasitize a high number of vertebrates, including domestic mammals and humans, where they are the main vectors of Rickettsia parkeri s.s. The identification, distribution, and validity of the three species within the group have been controversial and continue to raise questions. Previous studies have mostly focused on the analysis of partial nuclear and mitochondrial genes, highlighting the need for new integrative analyses that contribute to clarifying their systematics and ecology. In this investigation, we obtained the first mitochondrial genome of A. triste (14,808 bp), which, upon comparison with the reported mitochondrial genomes of A. maculatum, exhibits a genetic distance of 4.2%, providing new evidence for the validity of the former species. The order, composition, and structure of the mitogenome of A. triste are consistent with the characteristics reported for most Metastriata including taxa within Amblyomma, that count with several mitochondrial genomes published in the last few years. The attributes of the mitogenomes, such as the absence of paralogs and a small genome, confirm the utility in studies involving complexes of cryptic species as it also serves as a robust tool for inferring phylogenies. Furthermore, we reported new molecular markers at the mitochondrial level which can be used in phylogenetic studies for other tick species, especially those with controversial or challenging taxonomy. We highlight the need of sequencing the mitochondrial genome of A. tigrinum, which is part of the A. maculatum group, as well as experimental crosses of populations from different places in the Americas to obtain additional evidence for species recognition.","PeriodicalId":51306,"journal":{"name":"Systematic and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First mitochondrial genome of Amblyomma triste Koch, 1844 (Acari: Ixodidae): Evidence for studying species within the A. maculatum group\",\"authors\":\"Paula A. Ossa-lópez, J. Uribe, H. Ramírez-chaves, F. A. Rivera-Páez\",\"doi\":\"10.11158/saa.29.1.7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The tick species that comprise the Amblyomma maculatum group (Acari: Ixodidae) are widely distributed in the Nearctic and Neotropical regions, ranging from the United States of America (USA) to Argentina. This group includes three species: Amblyomma maculatum, Amblyomma tigrinum, and Amblyomma triste, which parasitize a high number of vertebrates, including domestic mammals and humans, where they are the main vectors of Rickettsia parkeri s.s. The identification, distribution, and validity of the three species within the group have been controversial and continue to raise questions. Previous studies have mostly focused on the analysis of partial nuclear and mitochondrial genes, highlighting the need for new integrative analyses that contribute to clarifying their systematics and ecology. In this investigation, we obtained the first mitochondrial genome of A. triste (14,808 bp), which, upon comparison with the reported mitochondrial genomes of A. maculatum, exhibits a genetic distance of 4.2%, providing new evidence for the validity of the former species. The order, composition, and structure of the mitogenome of A. triste are consistent with the characteristics reported for most Metastriata including taxa within Amblyomma, that count with several mitochondrial genomes published in the last few years. The attributes of the mitogenomes, such as the absence of paralogs and a small genome, confirm the utility in studies involving complexes of cryptic species as it also serves as a robust tool for inferring phylogenies. Furthermore, we reported new molecular markers at the mitochondrial level which can be used in phylogenetic studies for other tick species, especially those with controversial or challenging taxonomy. 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First mitochondrial genome of Amblyomma triste Koch, 1844 (Acari: Ixodidae): Evidence for studying species within the A. maculatum group
The tick species that comprise the Amblyomma maculatum group (Acari: Ixodidae) are widely distributed in the Nearctic and Neotropical regions, ranging from the United States of America (USA) to Argentina. This group includes three species: Amblyomma maculatum, Amblyomma tigrinum, and Amblyomma triste, which parasitize a high number of vertebrates, including domestic mammals and humans, where they are the main vectors of Rickettsia parkeri s.s. The identification, distribution, and validity of the three species within the group have been controversial and continue to raise questions. Previous studies have mostly focused on the analysis of partial nuclear and mitochondrial genes, highlighting the need for new integrative analyses that contribute to clarifying their systematics and ecology. In this investigation, we obtained the first mitochondrial genome of A. triste (14,808 bp), which, upon comparison with the reported mitochondrial genomes of A. maculatum, exhibits a genetic distance of 4.2%, providing new evidence for the validity of the former species. The order, composition, and structure of the mitogenome of A. triste are consistent with the characteristics reported for most Metastriata including taxa within Amblyomma, that count with several mitochondrial genomes published in the last few years. The attributes of the mitogenomes, such as the absence of paralogs and a small genome, confirm the utility in studies involving complexes of cryptic species as it also serves as a robust tool for inferring phylogenies. Furthermore, we reported new molecular markers at the mitochondrial level which can be used in phylogenetic studies for other tick species, especially those with controversial or challenging taxonomy. We highlight the need of sequencing the mitochondrial genome of A. tigrinum, which is part of the A. maculatum group, as well as experimental crosses of populations from different places in the Americas to obtain additional evidence for species recognition.
期刊介绍:
Systematic and Applied Acarology (SAA) is an international journal of the Systematic and Applied Acarology Society (SAAS). The journal is intended as a publication outlet for all acarologists in the world.
There is no page charge for publishing in SAA. If the authors have funds to publish, they can pay US$20 per page to enable their papers published for open access.
SAA publishes papers reporting results of original research on any aspects of mites and ticks. Due to the recent increase in submissions, SAA editors will be more selective in manuscript evaluation: (1) encouraging more high quality non-taxonomic papers to address the balance between taxonomic and non-taxonomic papers, and (2) discouraging single species description (see new special issues for single new species description) while giving priority to high quality systematic papers on comparative treatments and revisions of multiple taxa. In addition to review papers and research articles (over 4 printed pages), we welcome short correspondence (up to 4 printed pages) for condensed version of short papers, comments on other papers, data papers (with one table or figure) and short reviews or opinion pieces. The correspondence format will save space by omitting the abstract, key words, and major headings such as Introduction.