{"title":"小麦矮小病毒（Geminiviridae：Mastrevirus）截短启动子在改善水稻转基因表达方面的潜力","authors":"Marzieh Taghi-Malekshahi, Khalil Alami-Saeid, Esmaeel Ghasemi-Goojani, Mohamad Hamed Ghodoum Parizipour","doi":"10.1007/s40003-023-00689-0","DOIUrl":null,"url":null,"abstract":"<div><p><i>Wheat dwarf virus</i> (WDV) is a monocotyledonous plant-infecting virus that causes economical loss to cereal cultivations annually. The objective of this study was to use <i>Cauliflower mosaic virus</i> 35S promoter in combination with WDV large intergenic region (LIR) in rice (<i>Oryza sativa</i> L.) plant. Herein, a partial sequence from the genome of an Iranian isolate of WDV was used to synthesize a promoter for improvement of transgene expression in rice cultivars including Champa, Hashemi and Najafi. The promoter was cloned into pCAMBIA1304 and transferred into rice cells using <i>in planta</i> method. PCR, hygromycin resistance and in vivo expression of green fluorescent protein (mGFP5) were used for evaluating the efficiency of the synthesized promoter. The results showed that the WDV LIR can act as an efficient promoter for transgene expression in rice plant. Moreover, a significant difference was found between transformation rates in different rice cultivars; thus, Champa and Najafi cultivars showed the highest and lowest rate of transformation, respectively. WDV LIR was used to design a novel promoter for gene expression in rice cells. PCR assay, phenotypic experiment (antibiotic selection and GFP expression) and nucleotide sequencing showed that LIR is a promising source for the improvement of transgene expression within cells of monocotyledonous plants.</p></div>","PeriodicalId":7553,"journal":{"name":"Agricultural Research","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Potential of Wheat dwarf virus (Geminiviridae: Mastrevirus) Truncated Promoter for Improvement of Transgene Expression in Rice\",\"authors\":\"Marzieh Taghi-Malekshahi, Khalil Alami-Saeid, Esmaeel Ghasemi-Goojani, Mohamad Hamed Ghodoum Parizipour\",\"doi\":\"10.1007/s40003-023-00689-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Wheat dwarf virus</i> (WDV) is a monocotyledonous plant-infecting virus that causes economical loss to cereal cultivations annually. The objective of this study was to use <i>Cauliflower mosaic virus</i> 35S promoter in combination with WDV large intergenic region (LIR) in rice (<i>Oryza sativa</i> L.) plant. Herein, a partial sequence from the genome of an Iranian isolate of WDV was used to synthesize a promoter for improvement of transgene expression in rice cultivars including Champa, Hashemi and Najafi. The promoter was cloned into pCAMBIA1304 and transferred into rice cells using <i>in planta</i> method. PCR, hygromycin resistance and in vivo expression of green fluorescent protein (mGFP5) were used for evaluating the efficiency of the synthesized promoter. The results showed that the WDV LIR can act as an efficient promoter for transgene expression in rice plant. Moreover, a significant difference was found between transformation rates in different rice cultivars; thus, Champa and Najafi cultivars showed the highest and lowest rate of transformation, respectively. WDV LIR was used to design a novel promoter for gene expression in rice cells. PCR assay, phenotypic experiment (antibiotic selection and GFP expression) and nucleotide sequencing showed that LIR is a promising source for the improvement of transgene expression within cells of monocotyledonous plants.</p></div>\",\"PeriodicalId\":7553,\"journal\":{\"name\":\"Agricultural Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-01-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s40003-023-00689-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Research","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s40003-023-00689-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
Potential of Wheat dwarf virus (Geminiviridae: Mastrevirus) Truncated Promoter for Improvement of Transgene Expression in Rice
Wheat dwarf virus (WDV) is a monocotyledonous plant-infecting virus that causes economical loss to cereal cultivations annually. The objective of this study was to use Cauliflower mosaic virus 35S promoter in combination with WDV large intergenic region (LIR) in rice (Oryza sativa L.) plant. Herein, a partial sequence from the genome of an Iranian isolate of WDV was used to synthesize a promoter for improvement of transgene expression in rice cultivars including Champa, Hashemi and Najafi. The promoter was cloned into pCAMBIA1304 and transferred into rice cells using in planta method. PCR, hygromycin resistance and in vivo expression of green fluorescent protein (mGFP5) were used for evaluating the efficiency of the synthesized promoter. The results showed that the WDV LIR can act as an efficient promoter for transgene expression in rice plant. Moreover, a significant difference was found between transformation rates in different rice cultivars; thus, Champa and Najafi cultivars showed the highest and lowest rate of transformation, respectively. WDV LIR was used to design a novel promoter for gene expression in rice cells. PCR assay, phenotypic experiment (antibiotic selection and GFP expression) and nucleotide sequencing showed that LIR is a promising source for the improvement of transgene expression within cells of monocotyledonous plants.
期刊介绍：
The main objective of this initiative is to promote agricultural research and development. The journal will publish high quality original research papers and critical reviews on emerging fields and concepts for providing future directions. The publications will include both applied and basic research covering the following disciplines of agricultural sciences: Genetic resources, genetics and breeding, biotechnology, physiology, biochemistry, management of biotic and abiotic stresses, and nutrition of field crops, horticultural crops, livestock and fishes; agricultural meteorology, environmental sciences, forestry and agro forestry, agronomy, soils and soil management, microbiology, water management, agricultural engineering and technology, agricultural policy, agricultural economics, food nutrition, agricultural statistics, and extension research; impact of climate change and the emerging technologies on agriculture, and the role of agricultural research and innovation for development.
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