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引用次数: 0
摘要
本研究于 2021 年至 2023 年在拉胡里 MPKV 的植物病理学和农业微生物学系进行，目的是鉴定和筛选来自马哈拉施特拉邦不同地区不同旱生植物的不同氮杆菌和 PSB 分离物。在体外条件下，对 68 株氮核杆菌分离物进行了生化鉴定和筛选，以确定其促进植物生长的特性。对这些分离菌进行了生化鉴定，并在体外条件下对其促进植物生长的特性进行了筛选。利用随机扩增多态 DNA 分析法研究了分离菌株的 DNA 多态性。通过 RAPD 分析评估了细菌菌株之间的遗传多样性。所有分离菌株都显示出可重复的 DNA 带状模式。细菌菌株之间的多样性是根据条带大小数量和强度的变化来评估的。
Molecular Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India
The present study was conducted at Department of Plant Pathology and Agricultural Microbiology, MPKV, Rahuri during year 2021 to 2023 to characterize and screen different isolates of Azotobacter and PSB from Different Xerophytic plants from Different districts of Maharashtra State. 68 Azotobacter isolates were biochemically characterized and screened under In vitro conditions for their plant growth promoting properties. This isolates were biochemically characterized and screened In-vitro condition for their plant growth promoting properties. DNA polymorphism of isolates was studied using randomly amplified polymorphic DNA analysis. The genetic diversity among the bacterial strains was assessed by RAPD analysis. All the isolates showed reproducible DNA banding pattern. Diversity among the bacterial strains was assessed on the basis of variation of size number and intensity of bands.
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