利用内生真菌抑制 Ustilaginoidea virens (Cooke) Takahashi 在水稻植株中引起假烟雾病的生长

I. Sudarma, N. Suniti, Ni Nengah Darmiati
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摘要

水稻假烟熏病是由高桥真菌(Ustilaginoidea virens (Cooke) Takahashi)引起的,是一种田间偶发病原菌,症状是谷粒发黄,随着时间的推移变黑。叶片上的内生真菌有 15 个分离株,其中黄曲霉的感染率最高,为 40%;果实上的内生真菌感染率最高,为 80%;茎干上的内生真菌感染率最高,为 55%。叶片内生真菌的多样性指数(H')为 1.332,均匀一致指数(E)为 0.272,优势指数(D)为 0.72。果实内生真菌的 H'指数为 0.500,E 值为 0.273,D 值为 0.32。茎内生菌的 H' 指数为 0.959,E 值为 0.254,D 值为 0.58。叶片上的内生真菌抑制试验对黄曲霉的抑制率为 80 ± 0.2%,对根瘤菌的抑制率为 70 ± 0.3%。果实上的内生真菌只有黄曲霉,抑制率为 78 ± 0.5%,而茎上的内生真菌对黄曲霉和根瘤菌的抑制率分别为 80 ± 0.4% 和 80 ± 1.2%。
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Utilization of Endophites Fungi in Inhibiting the Growth of Ustilaginoidea virens (Cooke) Takahashi Causes False Smut Disease in Rice Plants invitro
False smut disease in rice is caused by the fungus Ustilaginoidea virens (Cooke) Takahashi, a sporadic pathogen attack in the field, with symptoms of yellow grain which turns black over time. The number of endophytic fungi on the leaves was 15 isolates with the highest prevalence, namely Aspergillus flavus of 40%, as well as the highest endophytic on fruit by 80% and the highest endophytic on stem by Rhizopus sp. by 55%. The diversity index (H') of endophytic fungi on leaves was 1.332, the evenness uniformity index (E) was 0.272 and the dominance index (D) was 0.72. In fruit endophytes the H' index was 0.500, the E value was 0.273 and the D value was 0.32. For endophytic stems, the H' value was 0.959, the E value was 0.254 and the D value was 0.58. Inhibition test of endophytic fungi on leaves only inhibited A. flavus by 80 ± 0.2% and Rhizopus sp. by 70 ± 0.3%. The endophytic fungi on fruit were only A. flavus which inhibited by 78 ± 0.5%, while the inhibition of endophytic fungi on stems, A. flavus and Rhizopus sp. can inhibit each by 80 ± 0.4% and 80 ± 1.2%.
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