EVALUATION OF IN VIVO ANTI-ACNE ACTIVITY OF FLOWER EXTRACT OF WITHANIA COAGULANS

Acne vulgaris is the widespread chronic skin illness. Conventional therapy has drawbacks, such as increasing antibiotic resistance in the microorganisms that cause acne. Herbs are a natural medicine source that has negligible to no adverse effects when used to treat acne. Numerous researches are carried out to help teens and other individuals avoid acne. Thus, this study deals with discovering the anti-acne potential of medicinal plants W. coagulans. Initially gathering of plant flowers, its extraction and qualitative and quantitative analysis was performed. The in vivo anti-acne activity was performed by inducing the heat killed P. acne in rats’ ear and measuring its thickness on specific days. The results showed that plant contains the alkaloid, flavonoid, phenol, protein, carbohydrate, saponin, diterpene, tannin. Total flavonoids content and Total phenol content were found to be0.67 and 0.95 mg/ 100 mg of dried extract of Withania coagulans respectively. In animal mode, at the end of 10th day the ear thickness in Withania coagulans 200 mg/kg treated rats was observed to be 0.19±0.05 which is near to ear thickness of 0.10±0.03 observed in Clindamycin 200 mg/kg p.o treated rats. The results of the extracts were comparable with standard. The data resulted from anti-acne effect of hydroalcoholic extract of Withania coagulans was significantly decreased the inflammation in rat’s ear. Thus, it can be concluded that that Withania coagulans had appreciable anti-acne activity. Withania coagulans can thus be utilized to develop antiacne formulations and tested in preclinical and clinical studies due to its good potency against bacteria that cause acne. KEYWORDS: Acne, Withania coagulans flowers, Phytochemicals, P. acne, Clindamycin, Ear thickness.