Determination of Phenolics and Flavonoids in Ethanolic Extract of Rosa Centifolia Using UV-Visible Spectroscopy

Objective: Our study aimed to analyze the phenolic properties of phytochemicals in ethanolic rose extract (Rosa centifolia L.). UV-Visible spectroscopic methods were used to achieve this goal. UV-Vis spectra helped identify the polyphenols present in the tested extract compared with the literature data. A large correlation was found between the antioxidant potential of the plant material (rose petals) tested. Materials and Methods: The extraction process itself, as well as the processing of rose petals, has undoubtedly significantly reduced the antioxidants present in it. Rose petals were extracted by distillation method using a soxhlet apparatus for 4 hours and fractionated by using various solvent systems, petroleum ether: chloroform, chloroform: ethyl acetate, and ethyl acetate: ethanol of increasing polarity (100:0 to 0:100 with constant polarity increase of 10%) 10 fractions of each solvent system. Results: The ethanolic extract UV-visible spectroscopy profile showed peaks at 268, 270, 285, 401, and 408 nm with the absorption 0.0128, 0.0126, 0.0051, 0.162, and 0.142 respectively. The final fraction of the extract shows 255, 257, 260, 273, 307, 401, and 408 nm with the absorption 0.0190, 0.017, 0.0399, 0.0279, 0.0181, 0.354, and 0.250 respectively. Conclusion: The analysis of the sample shows a noticeable presence of polyphenolic compounds Rose petal extracts can be an alternative to synthetic compounds in cosmetics, food, or medications, such as Trolox, BHA, and BHT.