{"title":"印度金丝夜蛾 Antheraea assamensis Helfer(鞘翅目,鳞翅目)的基因组测序与组装","authors":"Himanshu Dubey , A.R. Pradeep , Kartik Neog , Rajal Debnath , P.J. Aneesha , Suraj Kumar Shah , Indumathi Kamatchi , K.M. Ponnuvel , A. Ramesha , Kunjupillai Vijayan , Upendra Nongthomba , Utpal Bora , Sivaprasad Vankadara , K.M. VijayaKumari , Kallare P. Arunkumar","doi":"10.1016/j.ygeno.2024.110841","DOIUrl":null,"url":null,"abstract":"<div><p>Muga silkworm (<em>Antheraea assamensis</em>), one of the economically important wild silkmoths, is unique among saturniid silkmoths. It is confined to the North-eastern part of India. Muga silk has the highest value among the other silks. Unlike other silkmoths, <em>A. assamensis</em> has a low chromosome number (<em>n</em> = 15), and ZZ/ZO sex chromosome system. Here, we report the first high-quality draft genome of <em>A. assamensis,</em> assembled by employing the Illumina and PacBio sequencing platforms. The assembled genome of <em>A. assamensis</em> is 501.18 Mb long, with 2697 scaffolds and an N50 of 683.23 Kb. The genome encompasses 18,385 protein-coding genes, 86.29% of which were functionally annotated. Phylogenetic analysis of <em>A. assamensis</em> revealed its divergence from other Antheraea species approximately 28.7 million years ago. Moreover, an investigation into detoxification-related gene families, CYP450, GST, and ABC-transporter, revealed a significant expansion in <em>A. assamensis</em> as compared to the <em>Bombyx mori</em>. This expansion is comparable to <em>Spodoptera litura</em>, suggesting adaptive responses linked to the polyphagous behavior observed in these insects. This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on <em>A. assamensis</em> and other <em>Antheraea</em> species along with comparative genomics analyses of Bombycoidea insects.</p></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0888754324000624/pdfft?md5=af3f32a6d6e33c171f70c33f0d778d52&pid=1-s2.0-S0888754324000624-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera)\",\"authors\":\"Himanshu Dubey , A.R. Pradeep , Kartik Neog , Rajal Debnath , P.J. Aneesha , Suraj Kumar Shah , Indumathi Kamatchi , K.M. Ponnuvel , A. Ramesha , Kunjupillai Vijayan , Upendra Nongthomba , Utpal Bora , Sivaprasad Vankadara , K.M. VijayaKumari , Kallare P. Arunkumar\",\"doi\":\"10.1016/j.ygeno.2024.110841\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Muga silkworm (<em>Antheraea assamensis</em>), one of the economically important wild silkmoths, is unique among saturniid silkmoths. It is confined to the North-eastern part of India. Muga silk has the highest value among the other silks. Unlike other silkmoths, <em>A. assamensis</em> has a low chromosome number (<em>n</em> = 15), and ZZ/ZO sex chromosome system. Here, we report the first high-quality draft genome of <em>A. assamensis,</em> assembled by employing the Illumina and PacBio sequencing platforms. The assembled genome of <em>A. assamensis</em> is 501.18 Mb long, with 2697 scaffolds and an N50 of 683.23 Kb. The genome encompasses 18,385 protein-coding genes, 86.29% of which were functionally annotated. Phylogenetic analysis of <em>A. assamensis</em> revealed its divergence from other Antheraea species approximately 28.7 million years ago. Moreover, an investigation into detoxification-related gene families, CYP450, GST, and ABC-transporter, revealed a significant expansion in <em>A. assamensis</em> as compared to the <em>Bombyx mori</em>. This expansion is comparable to <em>Spodoptera litura</em>, suggesting adaptive responses linked to the polyphagous behavior observed in these insects. This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on <em>A. assamensis</em> and other <em>Antheraea</em> species along with comparative genomics analyses of Bombycoidea insects.</p></div>\",\"PeriodicalId\":12521,\"journal\":{\"name\":\"Genomics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0888754324000624/pdfft?md5=af3f32a6d6e33c171f70c33f0d778d52&pid=1-s2.0-S0888754324000624-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0888754324000624\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888754324000624","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera)
Muga silkworm (Antheraea assamensis), one of the economically important wild silkmoths, is unique among saturniid silkmoths. It is confined to the North-eastern part of India. Muga silk has the highest value among the other silks. Unlike other silkmoths, A. assamensis has a low chromosome number (n = 15), and ZZ/ZO sex chromosome system. Here, we report the first high-quality draft genome of A. assamensis, assembled by employing the Illumina and PacBio sequencing platforms. The assembled genome of A. assamensis is 501.18 Mb long, with 2697 scaffolds and an N50 of 683.23 Kb. The genome encompasses 18,385 protein-coding genes, 86.29% of which were functionally annotated. Phylogenetic analysis of A. assamensis revealed its divergence from other Antheraea species approximately 28.7 million years ago. Moreover, an investigation into detoxification-related gene families, CYP450, GST, and ABC-transporter, revealed a significant expansion in A. assamensis as compared to the Bombyx mori. This expansion is comparable to Spodoptera litura, suggesting adaptive responses linked to the polyphagous behavior observed in these insects. This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on A. assamensis and other Antheraea species along with comparative genomics analyses of Bombycoidea insects.
期刊介绍:
Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation.
As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.