ARUNDINIS Apiospora,碳水化合物活性酶和次生代谢物的集合体

IF 5.2 1区 生物学 Q1 MYCOLOGY Ima Fungus Pub Date : 2024-04-07 DOI:10.1186/s43008-024-00141-0
Trine Sørensen, Celine Petersen, Asmus T. Muurmann, Johan V. Christiansen, Mathias L. Brundtø, Christina K. Overgaard, Anders T. Boysen, Rasmus D. Wollenberg, Thomas O. Larsen, Jens L. Sørensen, Kåre L. Nielsen, Teis E. Sondergaard
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引用次数: 0

摘要

Apiospora 属由丝状真菌组成,具有巨大的潜力,但其全部能力仍未被发现。在本研究中,我们首次展示了 Arundinis Apiospora 分离物的基因组组装,显示了一个高度完整和连续的组装,估计为 48.8 Mb,N99 为 3.0 Mb。我们的分析预测了总共 15,725 个基因,并对其中 13,619 个基因进行了功能注释,揭示了真菌能够产生大量碳水化合物活性酶(CAZymes)和次级代谢产物。通过转录组分析,我们观察到基因表达对不同生长培养基的不同反应,当真菌在干草培养基上培养时,与碳水化合物代谢相关的几个基因出现显著上调。最后,我们的代谢组分析揭示了真菌能够产生多种代谢产物。
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Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites
The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.
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来源期刊
Ima Fungus
Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍: The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue
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