鲭鱼新型免疫缺陷基因的特征揭示了抗菌和抗病毒防御能力

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2024-04-12 DOI:10.1002/aah.10216
Utsanee Pinkaew, Chanitcha Choolert, Akapon Vaniksampanna, Phongthana Pasookhush, Siwaporn Longyant, Parin Chaivisuthangkura
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引用次数: 0

摘要

目的我们试图从大宗淡水对虾(又称大河对虾)中鉴定和描述一种免疫缺陷(IMD)同源物。IMD是一种含死亡域的蛋白质,在IMD通路--无脊椎动物对病毒和细菌入侵最重要的反应机制之一--中作为适配蛋白发挥着至关重要的作用。方法:利用快速扩增互补DNA末端的方法,分离了M.rosenbergii的一个IMD同源基因(MrIMD)。结果MrIMD的开放阅读框由555个核苷酸组成,编码一个由184个氨基酸组成的蛋白质,其C端有一个保守的死亡结构域。MrIMD蛋白与其他甲壳类动物的IMD相似度为53-74%,其中与东方对虾的IMD相似度最高。基因表达分析表明,鳃组织中的 MrIMD mRNA 水平最高。受嗜水气单胞菌刺激后,肌肉、鳃和肠中的MrIMD明显上调,而血细胞和肝胰脏中的MrIMD则无明显差异。这些结果表明,MrIMD 基因在应对革兰氏阴性菌和病毒感染时可能会发挥不同的作用,在先天性免疫中扮演着重要角色,是防御细菌和病毒感染的重要关键分子。
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Characterization of a novel immune deficiency gene of Macrobrachium rosenbergii reveals antibacterial and antiviral defenses

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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