水螅与光自养藻类之间的共生关系是否为保护 DNA 免受环境胁迫的细胞毒性或基因毒性作用模式的损害提供了进化优势?

IF 2.7 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Bulletin of Environmental Contamination and Toxicology Pub Date : 2024-04-02 DOI:10.1007/s00128-024-03884-z
Davor Želježić, Goran Kovačević, Ana Matijević, Petra Korać, Katarina Caput Mihalić
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引用次数: 0

摘要

本文旨在评估绿色水螅(Hydra viridissima)与光自养藻类之间的共生合作是否比棕色水螅(Hydra oligactis)在保护 DNA 完整性方面具有更强的抗性。Norflurazon 浓度为 0.061 或 0.61 mg/L,紫外线-B 光 254 nm,0.023mWcm- 2 可单独或同时使用。通过碱性彗星试验评估原生 DNA 损伤,通过荧光染色评估细胞毒性。与对照组相比,0.61 mg L- 1 的 Norflurazon 会显著增加棕色水螅的 DNA 损伤(6.17 ± 0.6 μm,5.2 ± 1.7% vs. 2.9 ± 0.2 μm,1.2 ± 0.2%)。细胞毒性明显升高,棕色水螅的细胞毒性更高(25.7 ± 3.5% vs. 8.2 ± 0.2%)。紫外线-B 对棕色水螅的 DNA 有明显损伤(13.5 ± 1.0 μm,4.1 ± 1.0%)。同时暴露于紫外线-B 和氟虫腈会导致 DNA 协同损伤。与绿色水螅(34.7 ± 2.5%;24.2 ± 0.6%)相比,棕色水螅(78.3 ± 9.4%;56.4 ± 6.0%)出现细胞毒性和刺猬核的频率更高。事实证明,进化过程中建立的共生合作关系能够抵御细胞毒性/遗传毒性。
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Does the Symbiotic Relationship Between Hydra Viridissima and Photoautotrophic Alga Provide an Evolutionary Advantage in Protecting DNA against Damage by the Cytotoxic or Genotoxic Mode of Action of Environmental Stressors?

The aim of this paper was to evaluate whether symbiotic cooperation between green hydra (Hydra viridissima) and photoautotrophic alga gives higher resistance of the preservation of DNA integrity compared to brown hydra (Hydra oligactis). Norflurazon concentrations were 0.061 or 0.61 mg/L and UV-B light 254 nm, 0.023mWcm− 2 applied separately or simultaneously. By alkaline comet assay primary DNA damage was assessed and cytotoxicity by fluorescent staining. Norflurazon at 0.61 mg L− 1 significantly increased DNA damage in brown hydras compared to the control (6.17 ± 0.6 μm, 5.2 ± 1.7% vs. 2.9 ± 0.2 μm, 1.2 ± 0.2%). Cytotoxicity was significantly elevated, being higher in brown hydras (25.7 ± 3.5% vs. 8.2 ± 0.2%). UV-B irradiation induced significant DNA damage in brown hydras (13.5 ± 1.0 μm, 4.1 ± 1.0%). Simultaneous exposure to UV-B and norflurazon led to a synergistic DNA damaging. The frequency of cytotoxicity and hedgehog nucleoids was more pronounced in brown (78.3 ± 9.4%; 56.4 ± 6.0%) than in green hydras (34.7 ± 2.5%; 24.2 ± 0.6%). Evolutionary established symbiotic cooperation proved to provide resistance against cyto/genotoxicity.

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来源期刊
CiteScore
5.60
自引率
3.70%
发文量
230
审稿时长
1.7 months
期刊介绍: The Bulletin of Environmental Contamination and Toxicology(BECT) is a peer-reviewed journal that offers rapid review and publication. Accepted submissions will be presented as clear, concise reports of current research for a readership concerned with environmental contamination and toxicology. Scientific quality and clarity are paramount.
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