{"title":"二磷酸尿苷葡萄糖基转移酶对昆虫（鳞翅目）的苯丙菊酯抗性至关重要","authors":"Kai-yi Zheng, Xiao-ying Zhang, Fasihul Lisan, Wen-Qin Lai, Qiang Zhang, Jun-li Lv, Zhan-peng Lu, Sheng Qin, Xia Sun, Shang-zhi Zhang, Xue-yang Wang, Li-shang Dai, Mu-wang Li","doi":"10.1111/imb.12912","DOIUrl":null,"url":null,"abstract":"<p>The silkworm (<i>Bombyx mori</i>) is an important model lepidopteran insect and can be used to identify pesticide resistance-related genes of great significance for biological control of pests. Uridine diphosphate glucosyltransferases (UGTs), found in all organisms, are the main secondary enzymes involved in the metabolism of heterologous substances. However, it remains uncertain if silkworm resistance to fenpropathrin involves UGT. This study observes significant variations in <i>BmUGT</i> expression among <i>B. mori</i> strains with variable fenpropathrin resistance post-feeding, indicating <i>BmUGT</i>'s role in fenpropathrin detoxification. Knockdown of <i>BmUGT</i> with RNA interference and overexpression of <i>BmUGT</i> significantly decreased and increased BmN cell activity, respectively, indicating that <i>BmUGT</i> plays an important role in the resistance of silkworms to fenpropathrin. In addition, fenpropathrin residues were significantly reduced after incubation for 12 h with different concentrations of a recombinant BmUGT fusion protein. Finally, we verified the conservation of UGT to detoxify fenpropathrin in <i>Spodoptera exigua</i>: Its resistance to fenpropathrin decreased significantly after knocking down <i>SeUGT</i>. In a word, UGT plays an important role in silkworm resistance to fenpropathrin by directly degrading the compound, a function seen across other insects. The results of this study are of great significance for breeding silkworm varieties with high resistance and for biological control of pests.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Uridine diphosphate glucosyltransferase is vital for fenpropathrin resistance in Bombyx mori (Lepidoptera)\",\"authors\":\"Kai-yi Zheng, Xiao-ying Zhang, Fasihul Lisan, Wen-Qin Lai, Qiang Zhang, Jun-li Lv, Zhan-peng Lu, Sheng Qin, Xia Sun, Shang-zhi Zhang, Xue-yang Wang, Li-shang Dai, Mu-wang Li\",\"doi\":\"10.1111/imb.12912\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The silkworm (<i>Bombyx mori</i>) is an important model lepidopteran insect and can be used to identify pesticide resistance-related genes of great significance for biological control of pests. Uridine diphosphate glucosyltransferases (UGTs), found in all organisms, are the main secondary enzymes involved in the metabolism of heterologous substances. However, it remains uncertain if silkworm resistance to fenpropathrin involves UGT. This study observes significant variations in <i>BmUGT</i> expression among <i>B. mori</i> strains with variable fenpropathrin resistance post-feeding, indicating <i>BmUGT</i>'s role in fenpropathrin detoxification. Knockdown of <i>BmUGT</i> with RNA interference and overexpression of <i>BmUGT</i> significantly decreased and increased BmN cell activity, respectively, indicating that <i>BmUGT</i> plays an important role in the resistance of silkworms to fenpropathrin. In addition, fenpropathrin residues were significantly reduced after incubation for 12 h with different concentrations of a recombinant BmUGT fusion protein. Finally, we verified the conservation of UGT to detoxify fenpropathrin in <i>Spodoptera exigua</i>: Its resistance to fenpropathrin decreased significantly after knocking down <i>SeUGT</i>. In a word, UGT plays an important role in silkworm resistance to fenpropathrin by directly degrading the compound, a function seen across other insects. The results of this study are of great significance for breeding silkworm varieties with high resistance and for biological control of pests.</p>\",\"PeriodicalId\":13526,\"journal\":{\"name\":\"Insect Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-04-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/imb.12912\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/imb.12912","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Uridine diphosphate glucosyltransferase is vital for fenpropathrin resistance in Bombyx mori (Lepidoptera)
The silkworm (Bombyx mori) is an important model lepidopteran insect and can be used to identify pesticide resistance-related genes of great significance for biological control of pests. Uridine diphosphate glucosyltransferases (UGTs), found in all organisms, are the main secondary enzymes involved in the metabolism of heterologous substances. However, it remains uncertain if silkworm resistance to fenpropathrin involves UGT. This study observes significant variations in BmUGT expression among B. mori strains with variable fenpropathrin resistance post-feeding, indicating BmUGT's role in fenpropathrin detoxification. Knockdown of BmUGT with RNA interference and overexpression of BmUGT significantly decreased and increased BmN cell activity, respectively, indicating that BmUGT plays an important role in the resistance of silkworms to fenpropathrin. In addition, fenpropathrin residues were significantly reduced after incubation for 12 h with different concentrations of a recombinant BmUGT fusion protein. Finally, we verified the conservation of UGT to detoxify fenpropathrin in Spodoptera exigua: Its resistance to fenpropathrin decreased significantly after knocking down SeUGT. In a word, UGT plays an important role in silkworm resistance to fenpropathrin by directly degrading the compound, a function seen across other insects. The results of this study are of great significance for breeding silkworm varieties with high resistance and for biological control of pests.
期刊介绍：
Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins.
This includes research related to:
• insect gene structure
• control of gene expression
• localisation and function/activity of proteins
• interactions of proteins and ligands/substrates
• effect of mutations on gene/protein function
• evolution of insect genes/genomes, especially where principles relevant to insects in general are established
• molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations
• gene mapping using molecular tools
• molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects
Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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