缺乏蛋白质液相成分的神经末梢突触小泡储备库的组织结构

N. V. Nifantova, A. G. Shishkov, O. M. Korenkova, E. Sopova, L. Brodin, O. Shupliakov
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引用次数: 0

摘要

摘要-- 在哺乳动物基因组中,蛋白质嗜内酯蛋白 A 由三个基因(嗜内酯蛋白 A1、A2 和 A3)编码,在外源性和内源性吞食过程中调节突触囊泡的循环,它存在于突触囊泡(SVs)的储备池中,其功能尚不清楚。体外实验表明,内噬蛋白通过其 SH3 结构域的相互作用,将几种成分结合到蛋白液相中,从而将 SV 组织到储备池中。我们研究了删除编码内嗜蛋白及其结合伙伴之一达因明的基因对培养中的大脑皮质神经元形成的活体突触中 SVs 组织的影响。我们的实验表明,内嗜蛋白基因的缺失不会改变储备池中 SV 的密度。同时,dynamin 1 和 dynamin 3 基因的缺失会导致囊泡密度显著增加。我们认为,作为蛋白液相成分的其他含SH3域蛋白可补充内嗜蛋白在SV储备池中的功能。
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Organization of the Reserve Pool of Synaptic Vesicles in Nerve Terminals Lacking Protein Liquid Phase Components

The protein endophilin A, which in the mammalian genome is encoded by three genes, endophilin A1, A2, and A3, regulates the synaptic vesicle cycle during exo- and endocytosis, and it is present in the reserve pool of synaptic vesicles (SVs), where its function is unknown. In vitro experiments suggest that endophilin, via its SH3 domain interactions, incorporates several components into the protein liquid phase that organizes SVs in the reserve pool. We investigated the effect of deletion of the genes encoding endophilin and one of its binding partners, dynamin, on the organization of SVs in living synapses formed by cortical neurons in culture. Our experiments showed that deletion of endophilin genes does not change the density of SVs in the reserve pool. At the same time, the deletion of dynamin 1 and dynamin 3 genes leads to a significant increase in the vesicle density. We suggest that other SH3-domain-containing proteins, which are components of the protein liquid phase, complement the function of endophilin in the SV reserve pool.

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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
28
期刊介绍: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology   is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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