利用带有 X 射线跟踪功能的 SIMBA 胶囊,在空间和时间上对小肠微生物组进行精确分析

Gang Wang, Sharanya Menon, Lynn Wilsack, Renata Rehak, Lawrence Lou, Christian Turbide, Jeremie Auger, Annie Tremblay, Olivier Mathieu, Sylvie Binda, Thomas A Tompkins, Sabina Bruehlmann, Christopher N Andrews
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引用次数: 0

摘要

在研究健康和疾病中的小肠微生物群时,几乎没有微创的小肠(SI)管腔液体采样方法。为了解决缺乏工具和方法来研究难以进入的消化道区域的问题,Nimble Science 公司开发了一种完全自主的被动采样方法--小肠微生物组抽吸(SIMBATM)胶囊,用于方便、高质量和可靠的采样,以研究小肠内饮食与微生物群之间的相互作用。首先通过体外模拟试验验证了 SIMBA 胶囊的密封效果和微生物 DNA 保存能力。然后,对 20 名健康参与者进行了一项临床研究,以验证在干预前后使用 SIMBA 胶囊可靠地采集样本用于 SI 微生物组分析(NCT04489329)。简而言之,参与者在基线和 7 天后分别摄入含有鼠李糖 R0011 和长球菌 R0175 混合菌株的益生菌胶囊。在基线摄入 SIMBA 胶囊后,进行了多次低剂量 X 射线扫描,以跟踪采样位置。对与基线胶囊和干预胶囊相对应的粪便样本进行分析比较。SIMBA 胶囊在体外的表现表明,它具有无污染采样和保存微生物群落的潜力。在临床研究中,胶囊在收集 SI 内容物方面的表现安全可靠。X 射线跟踪证实,97.2% 的胶囊在到达结肠前完成了 SI 区域的样本采集。重要的是,我们的数据显示,胶囊在肠道的正确区域采样,而且基线 SIMBA 微生物组图谱与粪便微生物组图谱有显著差异。SIMBA 成功检测到了小肠中同时存在的益生菌干预,而粪便样本则无法检测到。SIMBA 胶囊采样位置的高准确性和密封效果使其成为临床试验中有用的研究工具,用于研究饮食与微生物群在健康和疾病中的相互作用,并最终用于影响 SIBO 等消化道疾病的临床诊断。
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Spatially and Temporally Precise Microbiome Profiling in the Small Intestine using the SIMBA Capsule with X-ray tracking
Few minimally invasive options for sampling the small intestinal (SI) luminal fluid exist to study the SI microbiota in health and disease. To address the lack of tools and methods to study GI regions that are difficult to access, Nimble Science developed a fully autonomous and passive sampling method, the Small Intestine MicroBiome Aspiration (SIMBATM) capsule, for convenient, high-quality, and reliable sampling to study the diet-microbiota interactions in the SI. The sealing efficacy and microbial DNA preservation capacity of the SIMBA capsules was first validated through in vitro simulation assays. Then, a clinical study was conducted with 20 healthy participants to validate the in vivo use of SIMBA capsules to reliably capture samples for SI microbiome analysis before and after an intervention (NCT04489329). Briefly, participants ingested the capsules at baseline and 7 days later, with a probiotic capsule containing a blend of L. rhamnosus R0011 and B. longum R0175. Following baseline SIMBA capsule ingestion, multiple low-dosage x-ray scans were performed to track the sampling location. Fecal samples corresponding with the baseline and intervention capsule were analyzed for comparison. The SIMBA capsules’ performance in vitro demonstrated the potential for contamination-free sampling with preservation of the microbial communities. Within the clinical study, the capsules performed safely and reliably for collection of SI content. X-ray tracking confirmed that 97.2% of the capsules completed sample collection in the SI regions before reaching the colon. Importantly, our data showed that the capsules sampled in the right area of the intestines and that baseline SIMBA microbiome profile is significantly different from fecal microbiome profile. SIMBA successfully detected a concurrent probiotic intervention in the small intestine, which was not detectable using stool samples. The high accuracy of sampling location and sealing efficacy of the SIMBA capsules makes them potentially useful research tools in clinical trials for studying diet-microbiota interactions in health and disease, and perhaps eventually for the clinical diagnosis of GI tract conditions affecting the SI such as SIBO.
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