{"title":"银-氧化石墨烯-氧化钴纳米复合材料对 MRC-5 和 HepG2 细胞系细胞毒性水平的影响及分子对接研究","authors":"","doi":"10.1007/s10876-024-02604-1","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>The cytotoxic properties of cobalt oxide (Co<sub>3</sub>O<sub>4</sub>) nanoparticles (NPs), in addition to graphene oxide (GO)-Co<sub>3</sub>O<sub>4</sub> and silver (Ag)-GO-Co<sub>3</sub>O<sub>4</sub> nanocomposites (NCs), were evaluated against both human healthy lung fibroblast (MRC-5) and hepatocellular carcinoma (HepG2) cell lines utilizing the XTT assay. The investigation revealed that synthesized Co<sub>3</sub>O<sub>4</sub> NPs and NCs (GO-Co<sub>3</sub>O<sub>4</sub> and Ag-GO-Co<sub>3</sub>O<sub>4</sub>) elicited significant cytotoxic responses in MRC-5 and HepG2 cell lines in a concentration-dependent manner. Through molecular docking analyses, it was observed that all fabricated nanomaterials exhibited DNA recognition <em>via</em> minor groove binding, with molecular affinities ranging from − 4.82 to -11.66 kcal/mol. Furthermore, the docking outcomes illustrated that the angular conformations of GO-Co<sub>3</sub>O<sub>4</sub> and Ag-GO-Co<sub>3</sub>O<sub>4</sub> conferred ‘shape-selective’ characteristics as DNA minor groove binders, leading to heightened cytotoxicity, particularly in the HepG2 cell line compared to the normal MRC-5 cell line.</p>","PeriodicalId":618,"journal":{"name":"Journal of Cluster Science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies\",\"authors\":\"\",\"doi\":\"10.1007/s10876-024-02604-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Abstract</h3> <p>The cytotoxic properties of cobalt oxide (Co<sub>3</sub>O<sub>4</sub>) nanoparticles (NPs), in addition to graphene oxide (GO)-Co<sub>3</sub>O<sub>4</sub> and silver (Ag)-GO-Co<sub>3</sub>O<sub>4</sub> nanocomposites (NCs), were evaluated against both human healthy lung fibroblast (MRC-5) and hepatocellular carcinoma (HepG2) cell lines utilizing the XTT assay. The investigation revealed that synthesized Co<sub>3</sub>O<sub>4</sub> NPs and NCs (GO-Co<sub>3</sub>O<sub>4</sub> and Ag-GO-Co<sub>3</sub>O<sub>4</sub>) elicited significant cytotoxic responses in MRC-5 and HepG2 cell lines in a concentration-dependent manner. Through molecular docking analyses, it was observed that all fabricated nanomaterials exhibited DNA recognition <em>via</em> minor groove binding, with molecular affinities ranging from − 4.82 to -11.66 kcal/mol. Furthermore, the docking outcomes illustrated that the angular conformations of GO-Co<sub>3</sub>O<sub>4</sub> and Ag-GO-Co<sub>3</sub>O<sub>4</sub> conferred ‘shape-selective’ characteristics as DNA minor groove binders, leading to heightened cytotoxicity, particularly in the HepG2 cell line compared to the normal MRC-5 cell line.</p>\",\"PeriodicalId\":618,\"journal\":{\"name\":\"Journal of Cluster Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cluster Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s10876-024-02604-1\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cluster Science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10876-024-02604-1","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies
Abstract
The cytotoxic properties of cobalt oxide (Co3O4) nanoparticles (NPs), in addition to graphene oxide (GO)-Co3O4 and silver (Ag)-GO-Co3O4 nanocomposites (NCs), were evaluated against both human healthy lung fibroblast (MRC-5) and hepatocellular carcinoma (HepG2) cell lines utilizing the XTT assay. The investigation revealed that synthesized Co3O4 NPs and NCs (GO-Co3O4 and Ag-GO-Co3O4) elicited significant cytotoxic responses in MRC-5 and HepG2 cell lines in a concentration-dependent manner. Through molecular docking analyses, it was observed that all fabricated nanomaterials exhibited DNA recognition via minor groove binding, with molecular affinities ranging from − 4.82 to -11.66 kcal/mol. Furthermore, the docking outcomes illustrated that the angular conformations of GO-Co3O4 and Ag-GO-Co3O4 conferred ‘shape-selective’ characteristics as DNA minor groove binders, leading to heightened cytotoxicity, particularly in the HepG2 cell line compared to the normal MRC-5 cell line.
期刊介绍：
The journal publishes the following types of papers: (a) original and important research;
(b) authoritative comprehensive reviews or short overviews of topics of current
interest; (c) brief but urgent communications on new significant research; and (d)
commentaries intended to foster the exchange of innovative or provocative ideas, and
to encourage dialogue, amongst researchers working in different cluster
disciplines.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
