Karl F Ziegler, Kunaal Joshi, Charles S Wright, Shaswata Roy, Will Caruso, Rudro R Biswas, Srividya Iyer-Biswas
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Scaling of stochastic growth and division dynamics: A comparative study of individual rod-shaped cells in the Mother Machine and SChemostat platforms
A variety of microfluidic device designs are widely used to study the behaviors of single bacterial cells between growth conditions, but a quantitative comparison of growth between different devices remained absent.
The authors developed a protocol to obtain side-by-side experiments using two different microfluidic approaches, finding that growth rates and interdivision times differ but are precisely compensated by the division ratio distribution.
Identical emergent simplicities govern stochastic intergenerational homeostasis of cell sizes across device and experimental configurations. This intercondition scaling law establishes a principled route to draw comparisons of stochastic growth and division dynamics across experimental modalities.
期刊介绍：
MBoC publishes research articles that present conceptual advances of broad interest and significance within all areas of cell, molecular, and developmental biology. We welcome manuscripts that describe advances with applications across topics including but not limited to: cell growth and division; nuclear and cytoskeletal processes; membrane trafficking and autophagy; organelle biology; quantitative cell biology; physical cell biology and mechanobiology; cell signaling; stem cell biology and development; cancer biology; cellular immunology and microbial pathogenesis; cellular neurobiology; prokaryotic cell biology; and cell biology of disease.
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