单细胞 RNA 测序揭示静止黑素细胞干细胞的分子异质性

IF 3.9 3区 医学 Q2 CELL BIOLOGY Pigment Cell & Melanoma Research Pub Date : 2024-04-13 DOI:10.1111/pcmr.13169
Joseph W. Palmer, Nilesh Kumar, Luye An, Andrew C. White, M. Shahid Mukhtar, Melissa L. Harris
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引用次数: 0

摘要

毛囊黑色素细胞干细胞(McSCs)是皮肤中的稀有细胞群,在全皮肤单细胞RNA测序(scRNA-seq)数据集中的代表性明显不足。我们采用细胞富集策略从成年雌性 C57BL/6J 小鼠的毛囊皮肤中分离出了 KIT+/CD45- 细胞,高分辨率地评估了静止毛囊干细胞(qMcSCs)的转录情况。通过这项评估,我们证实了现有的qMcSC亚群分子特征(如Kit+、Cd34+/-、Plp1+、Cd274+/-、Thy1+、Cdh3+/-),并鉴定出了新的qMcSC亚群,其中包括两个能以不同方式调节其免疫特权状态的亚群。在qMcSC亚群中,我们还预测了黑色素细胞分化潜能、神经嵴潜能和静止深度。综上所述,研究结果表明qMcSC群体是异质性的,未来重点调查qMcSC变化的研究应考虑亚群组成的变化。
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Molecular heterogeneity of quiescent melanocyte stem cells revealed by single-cell RNA-sequencing

Melanocyte stem cells (McSCs) of the hair follicle are a rare cell population within the skin and are notably underrepresented in whole-skin, single-cell RNA sequencing (scRNA-seq) datasets. Using a cell enrichment strategy to isolate KIT+/CD45− cells from the telogen skin of adult female C57BL/6J mice, we evaluated the transcriptional landscape of quiescent McSCs (qMcSCs) at high resolution. Through this evaluation, we confirmed existing molecular signatures for qMcCS subpopulations (e.g., Kit+, Cd34+/−, Plp1+, Cd274+/−, Thy1+, Cdh3+/−) and identified novel qMcSC subpopulations, including two that differentially regulate their immune privilege status. Within qMcSC subpopulations, we also predicted melanocyte differentiation potential, neural crest potential, and quiescence depth. Taken together, the results demonstrate that the qMcSC population is heterogeneous and future studies focused on investigating changes in qMcSCs should consider changes in subpopulation composition.

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来源期刊
Pigment Cell & Melanoma Research
Pigment Cell & Melanoma Research 医学-皮肤病学
CiteScore
8.90
自引率
2.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders
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