Itzel Santiago-Figueroa, Manases González-Cortazar, Julieta Gertrudis Estrada-Flores, Jorge Alfredo Cuéllar-Ordaz, María Eugenia López-Arellano, Francisco Javier González-Reyes, Agustín Olmedo-Juárez, Rosa Isabel Higuera-Piedrahita
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引用次数: 0
摘要
目的我们分析了青蒿和万寿菊组合中的活性提取物对寄生于羊身上的线虫--轮虫（Haemonchus contortus）可能具有的协同活性。方法我们在体外对轮虫的卵和感染性幼虫（L3）进行了研究。采用 SAS 9.1 的 Proc Probit 进行回归分析，确定致死浓度 (LC) 值 LC50 和 LC90。结果表明，所研究的两种植物对线虫卵和 L3 幼虫以及它们的组合都有很高的功效。卵孵化抑制率最高的是 50/50 的组合，幼虫死亡率最高的是 25% A. cina 和 75% T. lucida（浓度为 10 mg/mL）的组合。结论本文研究的这两种植物具有很高的生物活性和协同作用，可用作天然抗蠕虫药的替代品。
Synergistic Interaction Effect of Artemisia cina n-hexane Extract and Tagetes lucida Ethyl Acetate Extract on Haemonchus Contortus
Purpose
We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus, a nematode parasitising sheep.
Methods
The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus. The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC50 and LC90 to determine the synergistic effect.
Results
The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect.
Conclusion
The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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