Nuciferine 通过抑制人类关节炎衍生成纤维细胞样滑膜细胞的增殖和侵袭以及纠正 Th17/Treg 失衡,缓解胶原蛋白诱发的大鼠关节炎

IF 4 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE Chinese Journal of Natural Medicines Pub Date : 2024-04-01 DOI:10.1016/S1875-5364(24)60622-9
Hao WANG , Xiaolong GENG , Fangbin AI , Zhilun YU , Yan ZHANG , Beibei ZHANG , Cheng LV , Ruiyang GAO , Bei YUE , Wei DOU
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引用次数: 0

摘要

类风湿性关节炎(RA)是一种慢性自身免疫性疾病,以持续的滑膜炎症和关节退化为特征,给有效治疗方法的开发带来了挑战。荷叶中的一种生物碱--荷叶碱(Nuciferine)具有良好的抗炎和抗肿瘤作用,但其在治疗类风湿性关节炎方面的疗效仍有待探索。本研究调查了荷叶碱对 MH7A 细胞系(一种源自人类 RA 的成纤维细胞样滑膜细胞)的抗增殖作用,结果显示荷叶碱能够抑制细胞增殖、促进细胞凋亡、诱导细胞凋亡并导致 G1/S 期停滞。此外,核iferine 还能显著降低 MH7A 细胞的迁移和侵袭能力。在胶原诱导的关节炎(CIA)大鼠模型中进一步评估了褐藻素的治疗潜力,发现它能明显减轻关节肿胀、滑膜增生、软骨损伤和炎症浸润。Nuciferine 还能改善胶原蛋白诱导的骨侵蚀,降低促炎细胞因子和血清免疫球蛋白(IgG、IgG1、IgG2a),并恢复 CIA 大鼠脾脏中 T 辅助细胞(Th)17 和调节性 T 细胞之间的平衡。这些结果表明,核iferine 可降低 FLS 细胞的增殖和侵袭性,并纠正 CIA 大鼠 Th17/Treg 细胞的失衡,从而为治疗 RA 带来优势。
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Nuciferine alleviates collagen-induced arthritic in rats by inhibiting the proliferation and invasion of human arthritis-derived fibroblast-like synoviocytes and rectifying Th17/Treg imbalance

Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by persistent synovial inflammation and joint degradation, posing challenges in the development of effective treatments. Nuciferine, an alkaloid found in lotus leaf, has shown promising anti-inflammatory and anti-tumor effects, yet its efficacy in RA treatment remains unexplored. This study investigated the antiproliferative effects of nuciferine on the MH7A cell line, a human RA-derived fibroblast-like synoviocyte, revealing its ability to inhibit cell proliferation, promote apoptosis, induce apoptosis, and cause G1/S phase arrest. Additionally, nuciferine significantly reduced the migration and invasion capabilities of MH7A cells. The therapeutic potential of nuciferine was further evaluated in a collagen-induced arthritis (CIA) rat model, where it markedly alleviated joint swelling, synovial hyperplasia, cartilage injury, and inflammatory infiltration. Nuciferine also improved collagen-induced bone erosion, decreased pro-inflammatory cytokines and serum immunoglobulins (IgG, IgG1, IgG2a), and restored the balance between T helper (Th) 17 and regulatory T cells in the spleen of CIA rats. These results indicate that nuciferine may offer therapeutic advantages for RA by decreasing the proliferation and invasiveness of FLS cells and correcting the Th17/Treg cell imbalance in CIA rats.

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来源期刊
Chinese Journal of Natural Medicines
Chinese Journal of Natural Medicines INTEGRATIVE & COMPLEMENTARY MEDICINE-PHARMACOLOGY & PHARMACY
CiteScore
7.50
自引率
4.30%
发文量
2235
期刊介绍: The Chinese Journal of Natural Medicines (CJNM), founded and sponsored in May 2003 by China Pharmaceutical University and the Chinese Pharmaceutical Association, is devoted to communication among pharmaceutical and medical scientists interested in the advancement of Traditional Chinese Medicines (TCM). CJNM publishes articles relating to a broad spectrum of bioactive natural products, leading compounds and medicines derived from Traditional Chinese Medicines (TCM). Topics covered by the journal are: Resources of Traditional Chinese Medicines; Interaction and complexity of prescription; Natural Products Chemistry (including structure modification, semi-and total synthesis, bio-transformation); Pharmacology of natural products and prescription (including pharmacokinetics and toxicology); Pharmaceutics and Analytical Methods of natural products.
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