五种细胞抑制剂在黑头鲦鱼(Pimephales promelas)幼体中的毒性基因组学研究

IF 2.7 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Bulletin of Environmental Contamination and Toxicology Pub Date : 2024-04-21 DOI:10.1007/s00128-024-03896-9
DC Castañeda-Cortés, M Lefebvre-Raine, G Triffault‐Bouchet, VS Langlois
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引用次数: 0

摘要

本研究评估了五种细胞抑制剂(他莫昔芬、甲氨蝶呤、卡培他滨、环磷酰胺和ifosfamide)对黑头呆鱼(Pimephales promelas)幼虫的毒性基因组效应。受精后的卵在浓度不断增加的药物中暴露六天。测量了两种毒性基因生物标志物和四种甲状腺激素相关基因通路的表达水平。有趣的是,结果显示,五种细胞抑制剂的所有浓度都会影响两种毒性生物标志基因的转录水平。此外,甲状腺激素相关基因的表达水平与对照组不同,在接触环磷酰胺和伊福酰胺的幼虫中观察到的变化最为显著。虽然之前的一项研究发现细胞抑制剂对鱼类的形态没有影响,但这项研究表明,这五种细胞抑制剂会改变海鲈鱼微妙的分子反应,突出了在动物的整个生命周期中评估多生物水平终点的重要性,以了解细胞抑制剂和其他污染物潜在影响的全貌。
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Toxicogenomics of Five Cytostatics in Fathead Minnow (Pimephales promelas) Larvae

In this study, the toxicogenomic effects of five cytostatics (tamoxifen, methotrexate, capecitabine, cyclophosphamide, and ifosfamide) on fathead minnow (Pimephales promelas) larvae were evaluated. Post-fertilization eggs were exposed to increasing concentrations of the drugs for six days. The expression levels of two genetic biomarkers for toxicity and four thyroid hormone-related gene pathways were measured. Interestingly, the results showed that all concentrations of the five cytostatics affect the transcription levels of both toxicity biomarker genes. Additionally, the thyroid hormone-related genes had different expression levels than the control, with the most significant changes observed in those larvae exposed to cyclophosphamide and ifosfamide. While a previous study found no effects on fish morphology, this study suggests that the five cytostatics modify subtle molecular responses of P. promelas, highlighting the importance of assessing multibiological level endpoints throughout the lifecycle of animals to understand the full portrait of potential effects of cytostatics and other contaminants.

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来源期刊
CiteScore
5.60
自引率
3.70%
发文量
230
审稿时长
1.7 months
期刊介绍: The Bulletin of Environmental Contamination and Toxicology(BECT) is a peer-reviewed journal that offers rapid review and publication. Accepted submissions will be presented as clear, concise reports of current research for a readership concerned with environmental contamination and toxicology. Scientific quality and clarity are paramount.
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