Jungwhoi Lee, Woogwang Sim, Jungsul Lee, Jae-Hoon Kim
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引用次数: 0
摘要
本研究的目的是提供一个合理的背景，通过识别可溶性 VSTM2L 来沉默类似 V 集和跨膜结构域的 2（VSTM2L），从而对抗胆管癌。通过迭代患者分区（IPP）计算筛选出了针对胆管癌的治疗靶点，并通过体外和硅学分析进行了验证。VSTM2L被选为胆管癌的潜在治疗靶点。通过阻断细胞内信号通路，抑制 VSTM2L 的表达可显著降低胆管癌细胞的活力和存活率。硅学分析表明，VSTM2L影响了胆管癌细胞生长的正向调节。Liptak's z 值显示，VSTM2L 的表达会恶化胆管癌患者的预后。此外，与健康献血者相比，胆管癌患者全血中可溶性 VSTM2L 的检出率更高。我们的报告显示，VSTM2L可能是胆管癌的潜在治疗靶点和可溶性预后生物标志物。
VSTM2L is a promising therapeutic target and a prognostic soluble-biomarker in cholangiocarcinoma.
The aim of the present study is to provide a rational background for silencing the V-set and transmembrane domain containing 2 like (VSTM2L) in consort with recognising soluble VSTM2L against cholangiocarcinoma. A therapeutic target against cholangiocarcinoma was selected using iterative patient partitioning (IPP) calculation, and it was verified by in vitro and in silico analyses. VSTM2L was selected as a potential therapeutic target against cholangiocarcinoma. Silencing the VSTM2L expression significantly attenuated the viability and survival of cholangiocarcinoma cells through blockade of the intracellular signalling pathway. In silico analysis showed that VSTM2L affected the positive regulation of cell growth in cholangiocarcinoma. Liptak's z value revealed that the expression of VSTM2L worsened the prognosis of cholangiocarcinoma patients. In addition, soluble VSTM2L was significantly detected in the whole blood of cholangiocarcinoma patients compared with that of healthy donors. Our report reveals that VSTM2L might be the potential therapeutic target and a soluble prognostic biomarker against cholangiocarcinoma.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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