单链 DNA 对 DNA 稳定的纳米银团簇的惰性如何？案例研究。

IF 3 4区化学 Q3 CHEMISTRY, PHYSICAL ChemPhotoChem Pub Date : 2024-04-22 DOI:10.1002/cptc.202400014

Vanessa Rück, Cecilia Cerretani, Tom Vosch

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引用次数: 0

摘要

本文报告了一项案例研究，详细阐述了不同 DNA 链对近红外发光 DNA 稳定银纳米簇（DNA-AgNC）的影响。研究发现，单链 DNA 寡聚体的存在会对 (DNA)2[Ag16Cl2]8+ 的化学稳定性产生不利影响，根据 DNA 序列的不同，破坏程度也不同。为了提高 DNA-AgNC 的化学稳定性，我们采用了两种保护策略。首先，将裸 DNA 链与相应的互补序列杂交，大大降低了 (DNA)2[Ag16Cl2]8+ 的破坏程度，吸收光谱和发射光谱中的降幅都有所减小就证明了这一点。其次，用银阳离子钝化裸 DNA 寡聚体后，(DNA)2[Ag16Cl2]8+ 依然完好无损。因此，我们的研究提供了一种简便易行的方法来发现对预制 DNA-AgNCs 反应性较低的 DNA 序列，并为如何保护 DNA-AgNCs 免受裸 DNA 链的影响提供了思路。

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How Inert is Single-Stranded DNA Towards DNA-Stabilized Silver Nanoclusters? A Case Study

A case study, detailing the effect of different DNA oligomers on a NIR-emitting DNA-stabilized silver nanocluster (DNA-AgNC), is reported. The presence of single-stranded DNA oligomers was found to adversely affect the chemical stability of (DNA)₂[Ag₁₆Cl₂]⁸⁺ with distinct degrees of destruction depending on the DNA sequence. To increase the chemical stability of the DNA-AgNC, we implemented two protection strategies. First, hybridization of the bare DNA strands with the corresponding complementary sequences dramatically reduced the destruction of (DNA)₂[Ag₁₆Cl₂]⁸⁺, as demonstrated by the decreased drops in both the absorption and emission spectra. Secondly, saturation of the free DNA oligomers with silver cations left (DNA)₂[Ag₁₆Cl₂]⁸⁺ intact. Our investigation can thus provide an easy-to-implement approach to discover DNA sequences that are intrinsically less reactive towards preformed DNA-AgNCs, and give an idea on how to protect DNA-AgNCs from bare DNA strands.

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来源期刊

ChemPhotoChem Chemistry-Physical and Theoretical Chemistry

CiteScore

5.80

自引率

5.40%

发文量

165

期刊介绍： Light plays a crucial role in natural processes and leads to exciting phenomena in molecules and materials. ChemPhotoChem welcomes exceptional international research in the entire scope of pure and applied photochemistry, photobiology, and photophysics. Our thorough editorial practices aid us in publishing authoritative research fast. We support the photochemistry community to be a leading light in science. We understand the huge pressures the scientific community is facing every day and we want to support you. Chemistry Europe is an association of 16 chemical societies from 15 European countries. Run by chemists, for chemists—we evaluate, publish, disseminate, and amplify the scientific excellence of chemistry researchers from around the globe.