中国森林麝香鹿麝腺发育的单核转录组学和染色质可及性分析

IF 3.5 1区 生物学 Q1 ZOOLOGY Integrative zoology Pub Date : 2024-04-21 DOI:10.1111/1749-4877.12823
Chenmiao LIU, Tingting HONG, Chengcheng ZHAO, Tao XUE, Shuhui WANG, Zhanjun REN
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引用次数: 0

摘要

雄性森林麝香鹿(Moschus berezovskii)麝香腺分泌的麝香是药物和香水的重要成分。麝香的分泌依赖于麝香腺的成熟;然而,其发育机制仍然难以捉摸。本文利用单细胞多组 ATAC + 基因表达结合多项生物信息学分析,揭示了麝香腺发育的动态转录细胞图谱,并确定了影响其发育的关键基因和转录因子。确定了 12 种细胞类型,包括两种不同类型的尖突细胞(0 群和 10 群)。单核 RNA 和单核 ATAC 测序分析表明,与麝香分泌有关的七个核心靶基因(Hsd17b2、Acacb、Lss、Vapa、Aldh16a1、Aldh7a1、和 Sqle)受 12 个核心转录因子(FOXO1、CUX2、RORA、RUNX1、KLF6、MGA、NFIC、FOXO3、ETV5、NR3C1、HSF4 和 MITF)的调控。京都基因和基因组百科全书》富集分析表明,在尖顶细胞发育过程中,与麝香分泌相关的通路发生了显著变化。基因组变异分析还显示,某些与麝香分泌相关的通路在6岁的尖顶细胞中得到了富集。构建了尖顶细胞发育过程中的基因共表达网络,以准确了解转录因子、基因和通路之间的联系。最后,细胞间通讯分析表明,细胞间通讯参与了麝香腺的发育。这项研究为了解麝香腺发育过程中的变化和关键因素提供了重要依据,为研究麝香分泌机制和促进这一濒危物种的保护提供了宝贵资源。
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Single-nucleus transcriptomics and chromatin accessibility analysis of musk gland development in Chinese forest musk deer (Moschus berezovskii)

Musk secreted by male forest musk deer (Moschus berezovskii) musk glands is an invaluable component of medicine and perfume. Musk secretion depends on musk gland maturation; however, the mechanism of its development remains elusive. Herein, using single cell multiome ATAC + gene expression coupled with several bioinformatic analyses, a dynamic transcriptional cell atlas of musk gland development was revealed, and key genes and transcription factors affecting its development were determined. Twelve cell types, including two different types of acinar cells (Clusters 0 and 10) were identified. Single-nucleus RNA and single-nucleus ATAC sequencing analyses revealed that seven core target genes associated with musk secretion (Hsd17b2, Acacb, Lss, Vapa, Aldh16a1, Aldh7a1, and Sqle) were regulated by 12 core transcription factors (FOXO1, CUX2, RORA, RUNX1, KLF6, MGA, NFIC, FOXO3, ETV5, NR3C1, HSF4, and MITF) during the development of Cluster 0 acinar cells. Kyoto Encyclopedia of Genes and Genomes enrichment showed significant changes in the pathways associated with musk secretion during acinar cell development. Gene set variation analysis also revealed that certain pathways associated with musk secretion were enriched in 6-year-old acinar cells. A gene co-expression network was constructed during acinar cell development to provide a precise understanding of the connections between transcription factors, genes, and pathways. Finally, intercellular communication analysis showed that intercellular communication is involved in musk gland development. This study provides crucial insights into the changes and key factors underlying musk gland development, which serve as valuable resources for studying musk secretion mechanisms and promoting the protection of this endangered species.

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来源期刊
CiteScore
6.40
自引率
12.10%
发文量
81
审稿时长
>12 weeks
期刊介绍: The official journal of the International Society of Zoological Sciences focuses on zoology as an integrative discipline encompassing all aspects of animal life. It presents a broader perspective of many levels of zoological inquiry, both spatial and temporal, and encourages cooperation between zoology and other disciplines including, but not limited to, physics, computer science, social science, ethics, teaching, paleontology, molecular biology, physiology, behavior, ecology and the built environment. It also looks at the animal-human interaction through exploring animal-plant interactions, microbe/pathogen effects and global changes on the environment and human society. Integrative topics of greatest interest to INZ include: (1) Animals & climate change (2) Animals & pollution (3) Animals & infectious diseases (4) Animals & biological invasions (5) Animal-plant interactions (6) Zoogeography & paleontology (7) Neurons, genes & behavior (8) Molecular ecology & evolution (9) Physiological adaptations
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Issue Information cba-miR-222-3p involved in photoperiod-induced apoptosis in testes of striped hamsters by targeting TRAF7. Exploring riverine aquatic animal diversity and establishing aquatic ecological monitoring approaches tailored to the Qinling region via eDNA technology. Sympatric diversity pattern driven by the secondary contact of two deeply divergent lineages of the soybean pod borer Leguminivora glycinivorella. Unlocking agro-ecosystem sustainability: exploring the bottom-up effects of microbes, plants, and insect herbivores.
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