抗坏血酸是抗白色念珠菌炎症反应的调节剂。

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-04-17 DOI:10.2217/fmb-2023-0188

Noala Vicensoto Moreira Milhan, Aline da Graça Sampaio, C. Y. Koga-Ito, Angela Bruzzaniti

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引用次数: 0

摘要

目的：评估口腔角质细胞在维生素 C（Vit C）存在下的行为及其抗炎潜力。材料与方法：首先将口腔角质细胞暴露于 0.1-2.5 mM 的维生素 C，然后分别使用 MTS 检测法和 Ibidi 培养插片评估细胞的代谢活性和迁移。然后，用白色念珠菌挑战细胞，并通过 qPCR 分析炎症标记物。结果显示维生素 C 能延缓细胞在 48 和 72 小时内的迁移，并能下调 IL-8 和 IL-1β 基因。结论维生素 C 具有抗炎潜力，可以作为抗真菌治疗的一种有趣的辅助剂。

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Ascorbic acid as a modulator of inflammatory response against Candida albicans.

Aim: To evaluate the behavior of oral keratinocytes in the presence of Vitamin C (Vit C) and its anti-inflammatory potential. Materials & methods: Oral keratinocytes were initially exposed to 0.1-2.5 mM of Vit C and the metabolic activity and cell migration were evaluated using MTS assay and Ibidi culture inserts, respectively. After, the cells were challenged with Candida albicans and inflammatory markers were analyzed by qPCR. Results: The treatment was not cytotoxic, and the highest concentrations increased the metabolic activity at 24 h. Vit C delayed the cell migration at 48 and 72 h. Interestingly, it downregulated the genes IL-8 and IL-1β. Conclusion: Vit C could be an interesting adjuvant to anti-fungal treatment due to its anti-inflammatory potential.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.