Fushuai Gong, Xiangru Zhou, Wang Yu, Hongwei Xu, Xiao-fu Zhou
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It was discovered that external application of ABA lessened the degree of damage on the donor side and lessened the damage caused by UV-B stress on R. chrysanthum. Additionally, integrated metabolomics and transcriptomics were used to examine the changes in differentially expressed genes (DEGs) and differential metabolites (DMs) in R. chrysanthum in order to have a better understanding of the role that ABA plays in carotenoid accumulation. The findings indicated that the majority of DEGs were connected to carotenoid accumulation and ABA signaling sensing. To sum up, we proposed a method for R. chrysanthum carotenoid accumulation. UV-B stress activates ABA production, which then interacts with transcription factors to limit photosynthesis and accumulate carotenoids, such as MYB-enhanced carotenoid biosynthesis. 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引用次数: 0
摘要
杜鹃花(Rhododendron chrysanthum,R. chrysanthum)的生长会受到紫外线 B 的影响,因为紫外线 B 会破坏光合作用系统,促进类胡萝卜素的积累。然而,目前还不清楚 R. chrysanthum 如何修复光合系统以促进类胡萝卜素色素的形成。本研究确定了类胡萝卜素和脱落酸(ABA)的浓度。紫外线-B胁迫后,类胡萝卜素的含量明显增加,类胡萝卜素与脱落酸之间有很强的相关性。为了验证 ABA 对类胡萝卜素积累的调控作用,研究人员考察了 R. chrysanthum 的 OJIP 瞬时曲线的变化。研究发现,外部施加 ABA 可减轻供体侧的损伤程度,并减轻紫外线-B 胁迫对 R. Chrysanthum 造成的损伤。此外,为了更好地了解 ABA 在类胡萝卜素积累中的作用,研究人员还利用代谢组学和转录组学综合研究了 R. chrysanthum 中差异表达基因(DEGs)和差异代谢物(DMs)的变化。研究结果表明,大部分 DEGs 与类胡萝卜素积累和 ABA 信号传感有关。总之,我们提出了一种 R. Chrysanthum 类胡萝卜素积累的方法。紫外线-B胁迫激活ABA的产生,然后ABA与转录因子相互作用,限制光合作用并积累类胡萝卜素,如MYB增强类胡萝卜素的生物合成。这项研究表明,类胡萝卜素的积累可以减轻 R. chrysanthum 在紫外线-B 胁迫下受到的伤害,同时也为提高植物的类胡萝卜素含量提供了有益的建议。
Carotenoid Accumulation in the Rhododendron chrysanthum Is Mediated by Abscisic Acid Production Driven by UV-B Stress
Rhododendron chrysanthum (R. chrysanthum) development is hampered by UV-B sunlight because it damages the photosynthetic system and encourages the buildup of carotenoids. Nevertheless, it is still unclear how R. chrysanthum repairs the photosynthetic system to encourage the formation of carotenoid pigments. The carotenoid and abscisic acid (ABA) concentrations of the R. chrysanthum were ascertained in this investigation. Following UV-B stress, the level of carotenoids was markedly increased, and there was a strong correlation between carotenoids and ABA. The modifications of R. chrysanthum’s OJIP transient curves were examined in order to verify the regulatory effect of ABA on carotenoid accumulation. It was discovered that external application of ABA lessened the degree of damage on the donor side and lessened the damage caused by UV-B stress on R. chrysanthum. Additionally, integrated metabolomics and transcriptomics were used to examine the changes in differentially expressed genes (DEGs) and differential metabolites (DMs) in R. chrysanthum in order to have a better understanding of the role that ABA plays in carotenoid accumulation. The findings indicated that the majority of DEGs were connected to carotenoid accumulation and ABA signaling sensing. To sum up, we proposed a method for R. chrysanthum carotenoid accumulation. UV-B stress activates ABA production, which then interacts with transcription factors to limit photosynthesis and accumulate carotenoids, such as MYB-enhanced carotenoid biosynthesis. This study showed that R. chrysanthum’s damage from UV-B exposure was lessened by carotenoid accumulation, and it also offered helpful suggestions for raising the carotenoid content of plants.