Effect of Different Processing Temperature on Motility and Viability in Canary Sperm

Background: Canaries have attracted people’s attention with their colors, shapes and impressive songs. The fact that canaries are domesticated and easy to obtain has made these birds attractive for songbird studies. In our study, canary semen was examined at different temperature values. This study aims to determine the most efficient spermatozoa examination temperature in canaries. Methods: In this study, five different temperature values (36, 38, 40, 42 and 44°C) were determined. The study used 6 active male Gloster canaries in each group. After each semen collection application, a 3-day break was given for the birds to rest. Dulbecco’s Modified Eagle Medium (DMEM) expander, which is preferred by songbirds, was used in the research. The semen was diluted 1:2 in a water bath at specified temperatures (36, 38, 40, 42, 44°C) and immediately examined for motility and viability. Result: When the study results were evaluated, it was seen that the viability rate at 38°C was higher than the other groups and 40°C gave the highest results in terms of motility. In addition to the viability rate at 38°C, the swimming speed of spermatozoa was also found to be more stable over a wider area than other groups. As a result, based on the data obtained from the study, it can be said that the most appropriate temperature for motility and viability in canary spermatozoa is 38°C.